5A11 antigen is a cell recognition molecule which is involved in neuronal‐glial interactions in avian neural retina
Open Access
- 1 April 1993
- journal article
- research article
- Published by Wiley in Developmental Dynamics
- Vol. 196 (4), 252-262
- https://doi.org/10.1002/aja.1001960406
Abstract
In continuing efforts to identify cell-surface molecules involved in cell-cell interactions in the developing avian retina, we identified a monoclonal antibody, the 5A11 antibody, which possessed the ability to interfere with contact-dependent glial cell maturation in vitro. We sought to determine the molecular and biochemical identity of the glycoprotein recognized by this antibody, and using additional criteria, establish whether the 5All antigen is indeed a cell-recognition molecule in the developing retina. Immuno-histochemical analyses demonstrate that in the hatchling chick retina and in live cultures of embryonic retina cells, the 5A11 antigen is predominantly associated with Müller glial cells whereas little is observed on neuronal elements. Microsequencing of the major component isolated by immunoaffinity chromatography identifies the HT7 antigen (Seulberger et al.: EMBO Journal 9:2151–2158, 1990), a unique member of the immunoglobulin super gene family (IGSF), as a homologous if not identical protein to the 5A11 antigen. The HT7 antibody, furthermore, recognizes affinity purified 5A11 antigen, and both the HT7 antibody and additional probes generated against the 5A11 antigen recognize a major polypeptide of 45.5 kDa and a minor band of 69 kDa on Western blots of membrane preparations from neural retina. To verify that the 5A11 antigen mediates cell-cell recognition events in the developing neural retina, we examined the consequences of adding antibody to monolayer cultures of dissociated embryonic retina cells and to dissociated retina cells in rotation-mediated suspension culture. Addition of the 5A11 antibody to monolayer cultures results in alteration in the development of the stereotypic arrangement of neurons and glia characterized by a reduction in the number and complexity of neural extensions upon the glial-derived flat cells. Similarly, addition of antibodies generated against the 5A11 antigen to dissociated cells in rotation cultures significantly reduces retina cell reaggregation as monitored by computer-assisted image analysis of cell aggregate size. These data and the identification of the 5A11 antigen as a member of the IGSF establish a role for the 5A11 antigen as a novel recognition molecule in the developing neural retina.Keywords
This publication has 37 references indexed in Scilit:
- Neurothelin: an inducible cell surface glycoprotein of blood-brain barrier-specific endothelial cells and distinct neurons.The Journal of cell biology, 1990
- The role of cell adhesion molecules in neurite outgrowth on Müller cellsDevelopmental Biology, 1990
- Regulatory aspects of the in vitro development of retinal Müller glial cellsCell Differentiation, 1987
- Sulfated glucuronic acid-containing glycoconjugates are temporally and spatially regulated antigens in the developing mammalian nervous systemDevelopmental Biology, 1987
- Gliogenesis in the embryonic avian optic tectum: neuronal-glial interactions influence astroglial phenotype maturationDevelopmental Brain Research, 1987
- Vesicle interactions as a model for the retinal cell-cell recognition mediated by R-cogninCell Differentiation, 1985
- Comparative characterization of monoclonal antibodies to carbonic anhydraseBiochemical and Biophysical Research Communications, 1984
- Cell disorganization and malformation in neural retina caused by antibodies to R-cognin: ultrastructural studyCell Differentiation, 1984
- The developmental distribution of vimentin in the chick retinaDevelopmental Brain Research, 1983
- Induction of glutamine synthetase in embryonic neural retina: its suppression by the gliatoxic agent α-aminoadipic acidDevelopmental Brain Research, 1981