A Critical Role of the Nuclear Receptor HR3 in Regulation of Gonadotrophic Cycles of the Mosquito Aedes aegypti
Open Access
- 26 September 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (9), e45019
- https://doi.org/10.1371/journal.pone.0045019
Abstract
The orphan nuclear receptor HR3 is essential for developmental switches during insect development and metamorphosis regulated by 20-hydroxyecdysone (20E). Reproduction of female mosquitoes of the major vector of Dengue fever, Aedes aegypti, is cyclic because of its dependence on blood feeding. 20E is an important hormone regulating vitellogenic events in this mosquito; however, any role for HR3 in 20E-driven reproductive events has not been known. Using RNA interference (RNAi) approach, we demonstrated that Aedes HR3 plays a critical role in a timely termination of expression of the vitellogenin (Vg) gene encoding the major yolk protein precursor. It is also important for downregulation of the Target-of-Rapamycin pathway and activation of programmed autophagy in the Aedes fat body at the end of vitellogenesis. HR3 is critical in activating betaFTZ-F1, EcRB and USPA, the expressions of which are highly elevated at the end of vitellogenesis. RNAi depletion of HR3 (iHR3) prior to the first gonadotrophic cycle affects a normal progression of the second gonadotrophic cycle. Most of ovaries 24 h post second blood meal from iHR3 females in the second cycle were small with follicles that were only slightly different in length from of those of resting stage. In addition, these iHR3 females laid a significantly reduced number of eggs per mosquito as compared to those of iMal and the wild type. Our results indicate an important role of HR3 in regulation of 20E-regulated developmental switches during reproductive cycles of A. aegypti females.Keywords
This publication has 44 references indexed in Scilit:
- Distinct roles of isoforms of the heme-liganded nuclear receptor E75, an insect ortholog of the vertebrate Rev-erb, in mosquito reproductionMolecular and Cellular Endocrinology, 2012
- Nutritional and hormonal regulation of the TOR effector 4E‐binding protein (4E‐BP) in the mosquito Aedes aegyptiThe FASEB Journal, 2011
- Autophagy in Drosophila melanogasterBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2009
- Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesisThe Journal of cell biology, 2008
- An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegyptiProceedings of the National Academy of Sciences of the United States of America, 2008
- Effect of insulin and 20-hydroxyecdysone in the fat body of the yellow fever mosquito, Aedes aegyptiInsect Biochemistry and Molecular Biology, 2007
- Distinct roles of Broad isoforms in regulation of the 20-hydroxyecdysone effector gene, Vitellogenin, in the mosquito Aedes aegyptiMolecular and Cellular Endocrinology, 2007
- ATG1, an autophagy regulator, inhibits cell growth by negatively regulating S6 kinaseEMBO Reports, 2007
- The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC CoactivatorMolecular and Cellular Biology, 2006
- Role and Regulation of Starvation-Induced Autophagy in the Drosophila Fat BodyDevelopmental Cell, 2004