No Evidence for a Different RBE between Pulsed and Continuous 20 MeV Protons
- 1 November 2009
- journal article
- Published by Radiation Research Society in Radiation Research
- Vol. 172 (5), 567-574
- https://doi.org/10.1667/rr1539.1
Abstract
To obtain greater insight into the future potential of tumor radiotherapy using proton beams generated from high-intensity lasers, it is important to characterize the ionization quality of the new beams by measuring the relative biological effectiveness (RBE) under conditions where the full dose at one irradiation site will be deposited by a few proton pulses less than 1 ns in duration. HeLa cells attached to a Mylar foil were irradiated with 70 kV X rays to obtain a reference dose–response curve or with 3 Gy of 20 MeV protons at the Munich tandem accelerator (Garching), either using a continuous mode where a cell sample was irradiated within a 100-ms time span or using a pulsed mode where radiation was given in a single proton pulse of about 1 ns. After irradiation cytochalasin B was added; 24 h later cells were fixed and stained with acridine orange and micronuclei were counted. The X-ray dose–response curve for the production of micronuclei in HeLa cells followed a linear-quadratic model. The corresponding RBE values for 20 MeV protons in pulsed and continuous irradiation modes were 1.07 ± 0.08 and 1.06 ± 0.10 in the first proton experiment and 1.09 ± 0.08 and 1.05 ± 0.11 in the second, respectively. There was no evidence for a difference in the RBE for pulsed and continuous irradiation of HeLa cells with 20 MeV protons.Keywords
This publication has 17 references indexed in Scilit:
- Quantitative analysis of DNA-damage response factors after sequential ion microirradiationRadiation and Environmental Biophysics, 2008
- Microirradiation of cells with energetic heavy ionsRadiation and Environmental Biophysics, 2004
- Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiationToxicology Letters, 2003
- Enhanced Values of the RBE and H Ratio for Cytogenetic Effects Induced by Secondary Electrons from an X-Irradiated Gold SurfaceRadiation Research, 2002
- Proton radiobiology and uncertaintiesRadiation Measurements, 1999
- Chromosome aberration frequencies in human lymphocytes irradiated in a multi-layer array by protons with different LETInternational Journal of Radiation Biology, 1997
- Comparison of γ-ray induced dicentric yields in human lymphocytes measured by conventional analysis and FISHMutation Research Letters, 1995
- Identification of aneuploidy‐inducing agents using cytokinesis‐blocked human lymphocytes and an antikinetochore antibodyEnvironmental and Molecular Mutagenesis, 1989
- Chromosome aberrations induced by protons up to 31 MeV in cultured human cellsRadiation and Environmental Biophysics, 1981
- Multinucleate Cells and Micronucleus Formation in Cultured Human Cells Exposed to 12 MeV Protons and γ-raysInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1980