Antiallergic effect of Gami-hyunggyeyeongyotang on ovalbumin-induced allergic rhinitis in mouse and human mast cells
Open Access
- 1 April 2016
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of the Chinese Medical Association
- Vol. 79 (4), 185-194
- https://doi.org/10.1016/j.jcma.2015.08.012
Abstract
Nduced AR in BALB/C mice, through examination of allergic inflammatory response regulation, as well as examination of human mast cells (HMC-1). Methods Nasal symptoms were evaluated in the OVA-induced allergic rhinitis mouse model, and total immunoglobulin (Ig)E and OVA-specific IgE levels in serum were investigated. Eosinophil infiltration and thickness of the nasal mucosa, and levels of interleukin (IL)-1β and caspase-1 were also measured by immunohistochemistry. Additionally, the effect of GMHGYGT on the phorbol-12-myristate-13-acetate plus calcium ionophore A23187-induced phosphorylation of extracellular signal-regulated kinase, C-Jun N-terminal kinase and p38 in HMC-1 cells was investigated. Results GMHGYGT was demonstrated to have antiallergic effects on the nasal symptoms of the OVA-induced mouse model, decreasing serum levels of OVA-specific IgE and levels of the cytokines IL-5, IL-6, IL-1β, monocyte chemotactic protein-1, and macrophage inflammatory protein-2. GMHGYGT reduced the number of eosinophils in the nasal mucosa and thickness of the nasal septum, and inhibited the expression of IL-1β and caspase-1. Moreover, it inhibited the phosphorylation of extracellular signal-regulated kinase and C-Jun N-terminal kinase, as well as the activation of nuclear factor-κB on protein level in HMC-1 cells. Conclusion These results suggest that GMHGYGT has therapeutic potential for the treatment of allergic rhinitis....Keywords
Funding Information
- Hamsoa Pharmaceutical Co. R&D Center (20121084)
This publication has 27 references indexed in Scilit:
- Protective Effects of Exogenous Surfactant Protein A in Allergic Rhinitis: A Mouse ModelAnnals of Otology, Rhinology & Laryngology, 2013
- Allergic Rhinitis and Its Impact on Asthma in Asia Pacific and the ARIA Update 2008World Allergy Organization Journal, 2012
- The economic impact of allergic rhinitis and current guidelines for treatmentAnnals of Allergy, Asthma & Immunology, 2011
- Beneficial effects of chelidonic acid on a model of allergic rhinitisInternational Immunopharmacology, 2011
- Effect of oral tolerance in a mouse model of allergic rhinitisOtolaryngology -- Head and Neck Surgery, 2010
- Effect of Brazilian propolis on sneezing and nasal rubbing in experimental allergic rhinitis of miceImmunopharmacology and Immunotoxicology, 2009
- Acute uriticaria-like lesions in allergen-unexposed cutaneous tissues in a mouse model of late allergic rhinitisInternational Journal of Experimental Pathology, 2008
- Objective monitoring of nasal airway inflammation in rhinitisJournal of Allergy and Clinical Immunology, 2005
- The human mast cellJournal of Allergy and Clinical Immunology, 1997
- Nasal challenge with allergen upregulates the local expression of vascular endothelial adhesion moleculesJournal of Allergy and Clinical Immunology, 1994