Diversity and Relative Levels of Actinidin, Kiwellin, and Thaumatin-Like Allergens in 15 Varieties of Kiwifruit (Actinidia)
- 14 January 2013
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 61 (3), 728-739
- https://doi.org/10.1021/jf304289f
Abstract
In the last 30 years the incidence of kiwifruit allergy has increased with the three major allergenic proteins being identified as actinidin, kiwellin, and thaumatin-like protein (TLP). We report wide variation in the levels of actinidin and TLP in 15 kiwifruit varieties from the four most widely cultivated Actinidia species. Acidic and basic isoforms of actinidin were identified in Actinidia deliciosa ‘Hayward’ and Actinidia arguta ‘Hortgem Tahi’, while only a basic isoform of actinidin was identified in Actinidia chinensis ‘Hort16A’. One isoform each of kiwellin and TLP were identified in ripe fruit. The cysteine protease activity of actinidin correlated with protein levels in all species except A. arguta. Protein modeling suggested that modifications to the S2 binding pocket influenced substrate specificity of the A. arguta enzyme. Our results indicate that care is necessary when extrapolating allergenicity results from single varieties to others within the same and between different Actinidia species.This publication has 54 references indexed in Scilit:
- What is a food allergen?Clinical & Experimental Allergy, 2008
- The prevalence of plant food allergies: A systematic reviewJournal of Allergy and Clinical Immunology, 2008
- Kiwi fruit allergy: A reviewPediatric Allergy and Immunology, 2003
- Latex-fruit syndromeCurrent Allergy and Asthma Reports, 2003
- Determination and characterization of cross-reacting allergens in latex, avocado, banana, and kiwi fruit.Allergy, 1998
- Isolation and characterization of a major allergen in kiwi fruitZeitschrift Für Lebensmitteluntersuchung Und -Forschung A, 1997
- Characterization of allergens in kiwi fruit and detection of cross‐reactivities with allergens of birch pollen and related fruit allergensFood and Agricultural Immunology, 1997
- Identification of the allergenic components of kiwi fruit and evaluation of their cross-reactivity with timothy and birch pollensJournal of Allergy and Clinical Immunology, 1996
- Common allergenic structures in hazelnut, rye grain, sesame seeds, kiwi, and poppy seedsAllergy, 1993
- Hypersensitivity reaction to kiwi fruit (Chinese gooseberry, Actinidia chinensis)Journal of Allergy and Clinical Immunology, 1981