Cell Lineage Analysis by Intracellular Injection of Fluorescent Tracers
- 26 September 1980
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 209 (4464), 1538-1541
- https://doi.org/10.1126/science.6159680
Abstract
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.Keywords
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