Susceptibility of Prostate Epithelial Cells toChlamydia muridarumInfection and Their Role in Innate Immunity by Recruitment of Intracellular Toll-Like Receptors 4 and 2 and MyD88 to the Inclusion
- 1 December 2006
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 74 (12), 6973-6981
- https://doi.org/10.1128/iai.00593-06
Abstract
Although Chlamydia infections are widespread throughout the world, data about immunopathogenesis of genitourinary tract infections in males are very limited. In the present work we present an in vitro model of male genital tract-derived epithelial cells, more precisely prostate epithelial cells (PEC), to analyze if they are susceptible and able to respond to Chlamydia muridarum infection. Our results demonstrate that rat PEC are susceptible to C. muridarum infection and respond to this pathogen by up-regulating different proinflammatory cytokine and chemokine genes that could participate in the recruitment and local activation of immune cells, therefore influencing innate and adaptive immune responses during Chlamydia infection. Moreover, we analyzed the expression of Toll-like receptor 4 (TLR4), TLR2, and related molecules on PEC and the effect of C. muridarum infection on their expression. Our results demonstrate that PEC express significant levels of TLR4, CD14, TLR2, and the adaptor molecule MyD88 and up-regulate these proteins in response to C. muridarum infection. Indeed, TLR4, CD14, TLR2, and the adaptor MyD88 are specifically recruited to the vicinity of the bacterial inclusion, suggesting that these TLRs are actively engaged in signaling from this intracellular location in these cells. This is, to our knowledge, the first time that an in vitro model of infection with Chlamydia of male tract-derived epithelial cells has been achieved, and it provides the opportunity to determine how these cells respond and participate in modulating innate and adaptive immune response during Chlamydia infections.Keywords
This publication has 54 references indexed in Scilit:
- Prostate epithelial cells can act as early sensors of infection by up-regulating TLR4 expression and proinflammatory mediators upon LPS stimulationJournal of Leukocyte Biology, 2006
- Comparison of Gamma Interferon-Mediated Antichlamydial Defense Mechanisms in Human and Mouse CellsInfection and Immunity, 2006
- Chlamydia trachomatis: time for screening?Clinical Microbiology & Infection, 2005
- A role for surfactant protein D in innate immunity of the human prostateThe Prostate, 2005
- Ectopic Pregnancies and Reproductive Capacity After Chlamydia trachomatis Positive and Negative Test Results: A Historical Follow-Up StudySexually Transmitted Diseases, 2005
- Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccineNature Reviews Immunology, 2005
- Murine Oviduct Epithelial Cell Cytokine Responses toChlamydia muridarumInfection Include Interleukin-12-p70 SecretionInfection and Immunity, 2004
- Inflammatory-associated obstructions of the male reproductive tractAndrologia, 2003
- Chemokine and Chemokine Receptor Dynamics during Genital Chlamydial InfectionInfection and Immunity, 2002
- Establishment and characterization of seven dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancersThe Prostate, 1986