New polymorphic tetranucleotide microsatellites improve scoring accuracy in the bottlenose dolphin Tursiops aduncus
- 12 February 2009
- journal article
- permanent genetic-resources-note
- Published by Wiley in Molecular Ecology Resources
- Vol. 9 (2), 531-534
- https://doi.org/10.1111/j.1755-0998.2008.02246.x
Abstract
We isolated and characterized 19 novel tetranucleotide microsatellite markers in the Indo-Pacific bottlenose dolphin (Tursiops aduncus) in order to improve genotyping accuracy in applications like large-scale population-wide paternity and relatedness assessments. One hundred T. aduncus from Shark Bay, Western Australia, were screened for polymorphism. Cross-amplification was tested on four other small odontocete species. The new tetranucleotide microsatellite loci showed a more than fourfold higher scoring accuracy and significantly lower stutter formation compared to eight dinucleotide loci, although overall allelic diversity was significantly reduced.Keywords
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