Isolation of mixed subtypes of influenza A virus from a bald eagle (Haliaeetus leucocephalus)
Open Access
- 28 July 2010
- journal article
- Published by Springer Science and Business Media LLC in Virology Journal
- Vol. 7 (1), 174
- https://doi.org/10.1186/1743-422x-7-174
Abstract
From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool consisting of five samples. All pools (n = 50) were screened for the presence of avian influenza virus (AIV) using a real time reverse transcription-polymerase chain reaction (rRT-PCR); one of the pools was found positive. All five samples in this pool were tested individually by rRT-PCR; one sample from a bald eagle was found positive. This sample was inoculated in embryonated chicken eggs for virus isolation and a hemagglutinating virus was isolated. Complete genome sequencing of the isolate revealed a mixed infection with H1N1 and H2N1 subtypes. Further analysis revealed that the PB1-F2 gene sequence of H1N1 virus had the N66S virulence-associated substitution. Further studies on ecology and epidemiology of AIV in raptors are needed to help understand their role in the maintenance and evolution of AIV.Keywords
This publication has 17 references indexed in Scilit:
- The Feasibility of Using High Resolution Genome Sequencing of Influenza A Viruses to Detect Mixed Infections and QuasispeciesPLOS ONE, 2009
- Spatial, Temporal, and Species Variation in Prevalence of Influenza A Viruses in Wild Migratory BirdsPLoS Pathogens, 2007
- Surveillance of Influenza Virus A in Migratory Waterfowl in Northern EuropeEmerging Infectious Diseases, 2007
- Amplification of the entire genome of influenza A virus H1N1 and H3N2 subtypes by reverse-transcription polymerase chain reactionJournal of Virological Methods, 2006
- H5N1 virus outbreak in migratory waterfowlNature, 2005
- Highly Pathogenic H5N1 Influenza Virus in Smuggled Thai Eagles, BelgiumEmerging Infectious Diseases, 2005
- Influenza A Viruses of Migrating Wild Aquatic Birds in North AmericaVector-Borne and Zoonotic Diseases, 2004
- Long-term Monitoring for Avian Influenza Viruses in Wild Bird Species in ItalyVeterinary Research Communications, 2003
- Avian influenza virus (H7 serotype) in a saker falcon in Italy.2000
- Host range of avian influenza virus in free-living birdsVeterinary Research Communications, 1988