Luminescent Core–Shell Imprinted Nanoparticles Engineered for Targeted Förster Resonance Energy Transfer-Based Sensing
- 15 May 2013
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 85 (11), 5316-5320
- https://doi.org/10.1021/ac400520s
Abstract
Red-luminescent 200 nm silica nanoparticles have been designed and prepared as a versatile platform for developing FRET (Förster resonance energy transfer) biomimetic assays. Ru(phen)32+ dye molecules embedded off-center in the silica core provide the long-lived donor emission, and a near-infrared labeled analyte serves as fluorescent acceptor (the measured R0 of this D–A pair is 4.3 nm). A thin surface-grafted molecularly imprinted polymer (MIP) shell intervenes as selective enrofloxacin-binding element. These nanoparticles have been tested for photochemical detection of enrofloxacin by using a competitive scheme that can be readily performed in MeCN–HEPES (pH 7.5) 7:3 (v/v) mixtures and allows for the antibiotic detection in the μM range (LOD = 2 μM) without optimization of the assay. Given the well-known difficulties of coupling the target-binding-to-MIP and the transducing events, the novel photochemical approach tuned up here will be valuable in future developments of MIP-based assays and optosensors that capitalize also on the advantages of nanomaterials for (bio)analysis.Keywords
This publication has 31 references indexed in Scilit:
- Cubic Molecularly Imprinted Polymer Nanoparticles with a Fluorescent CoreAngewandte Chemie, 2012
- Molecular ImprintingPublished by Springer Science and Business Media LLC ,2012
- Composite QDs@MIP Nanospheres for Specific Recognition and Direct Fluorescent Quantification of Pesticides in Aqueous MediaAnalytical Chemistry, 2011
- Surface molecular imprinting onto fluorescein-coated magnetic nanoparticlesvia reversible addition fragmentation chain transfer polymerization: A facile three-in-one system for recognition and separation of endocrine disrupting chemicalsNanoscale, 2010
- Core-shell nanostructured molecular imprinting fluorescent chemosensor for selective detection ofatrazine herbicideThe Analyst, 2010
- Preparation of Surface Imprinting Polymer Capped Mn-Doped ZnS Quantum Dots and Their Application for Chemiluminescence Detection of 4-Nitrophenol in Tap WaterAnalytical Chemistry, 2010
- Molecularly Imprinted Silica Nanospheres Embedded CdSe Quantum Dots for Highly Selective and Sensitive Optosensing of PyrethroidsChemistry of Materials, 2010
- Fluorescent imprinted polymer sensors for chiral aminesOrganic & Biomolecular Chemistry, 2009
- Molecular Imprinting: Synthetic Materials As Substitutes for Biological Antibodies and ReceptorsChemistry of Materials, 2008
- Fluorescent Imprinted Polymers Prepared with 2-Acrylamidoquinoline as a Signaling MonomerOrganic Letters, 2005