Determination of α- and β-trenbolone in bovine muscle and liver by liquid chromatography with fluorescence detection
- 10 March 2000
- journal article
- research article
- Published by Elsevier BV in Journal of Chromatography B: Biomedical Sciences and Applications
- Vol. 739 (2), 363-367
- https://doi.org/10.1016/s0378-4347(99)00557-5
Abstract
A sensitive and rapid high-performance liquid chromatographic screening method is described for the determination of anabolic steroid trenbolone in bovine muscle and liver. Trenbolone was analyzed as alpha- and beta-trenbolone. Samples were extracted with ethyl acetate and cleaned up on a silica solid-phase extraction (SPE) cartridge. Liver samples were cleaned up on a multifunctional SPE cartridge before using a silica SPE cartridge. Analysis of alpha- and beta-trenbolone was performed by reversed-phase high-performance liquid chromatography (HPLC) with a fluorescence detector. The detection limits for this method were estimated to be 0.2 and 1.0 ng/g in bovine muscle and liver, respectively. The mean recoveries spiked in muscle at 2 ng/g and in liver at 10 ng/g were over 80%.Keywords
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