Platelet-Rich Plasma: Quantification of Growth Factor Levels and the Effect on Growth and Differentiation of Rat Bone Marrow Cells
- 1 November 2006
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 12 (11), 3067-3073
- https://doi.org/10.1089/ten.2006.12.3067
Abstract
Platelet-rich plasma (PRP) is a new application of tissue engineering and a developing area for clinicians and researchers. It is a storage vehicle of growth factors (GFs) such as platelet-derived growth factor (PDGF)- AA, -BB, -AB; transforming growth factor (TGF)-β1 and -2; platelet-derived epidermal growth factor (PDEGF); platelet-derived angiogenesis factor (PDAF); insulin growth factor-1 (IGF-1); and platelet factor- 4 (PF-4), which are known to influence bone regeneration. However, animal and clinical studies reveal different results with the use of PRP and its effect on bone healing. This could be due to the differences between species, that is, differences between species in GF concentrations or variation in presence of GFs between the various PRPs. In this study, rat bone marrow cells were cultured in PRP-coated wells or in uncoated wells for 16 days in osteogenic medium, and analyzed on cell growth (DNA content) and cell differentiation (alkaline phosphatase [ALP] activity, calcium content, scanning electron microscopy, and QPCR). The concentrations of TGF-β1, PDGF-AA, PDGF-AB, and PDGF-BB in rat, goat, and human PRP were subsequently determined. The results showed that PRP stimulated initial cell growth and had no effect on ALP activity. The calcium measurements showed a significant increase in calcium at days 8, 12, and 16. The real-time PCR results showed that PRP upregulated osteocalcin at day 1 and collagen type I at day 8. Overall, the immunoassays revealed that human PRP contained higher concentrations of growth factors per platelet compared to rat and goat PRP. Goat PRP showed higher concentrations of growth factors per platelet as compared to rat PRP except for PDGF-BB, which had a higher concentration in rat PRP. TGF-β1 was the most abundant growth factor in all 3 PRPs. On the basis of our results, we conclude that platelet-rich plasma contains osteo-inductive growth factors, which are probably species related. However, we cannot generalize the results because of large intraspecies variations. Further, we conclude that rat PRP gel stimulates initial growth and differentiation of rat bone marrow cells in vitro.Keywords
This publication has 26 references indexed in Scilit:
- Sinus floor elevation applied tissue‐engineered boneClinical Oral Implants Research, 2005
- Platelet-rich plasma: evidence to support its useJournal of Oral and Maxillofacial Surgery, 2004
- Effect of Platelet Releasate on Bone Cell Migration and Recruitment In VitroThe Journal of Craniofacial Surgery, 2003
- Platelet-derived growth factors enhance proliferation of human stromal stem cellsBiomaterials, 2003
- Investigation of platelet-rich plasma in rabbit cranial defects: A pilot studyJournal of Oral and Maxillofacial Surgery, 2002
- Platelets stimulate proliferation of bone cells: involvement of platelet‐derived growth factor, microparticles and membranesClinical Oral Implants Research, 2002
- Wachstumsstimulation von humanen osteoblastenähnlichen Zellen durch Thrombozytenkonzentrate in vitroMund-,Kiefer- und Gesichtschirurgie, 2002
- Signal Pathways That Transduce Growth Factor-Stimulated Mitogenesis in Bone CellsBone, 1998
- Platelet-rich plasmaOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 1998
- PDGF induces tyrosine phosphorylation in osteoblast-like cells: relevance to mitogenesisAmerican Journal of Physiology-Endocrinology and Metabolism, 1992