Food contaminant acrylamide increases expression of Cox-2 and nitric oxide synthase in breast epithelial cells
- 16 August 2010
- journal article
- research article
- Published by SAGE Publications in Toxicology and Industrial Health
- Vol. 27 (1), 11-18
- https://doi.org/10.1177/0748233710380217
Abstract
Acrylamide has been discovered in foods cooked at high temperature. A potentially harmful effect of this dietary component has been suggested by data indicating its association with increased breast cancer. This study investigated the potential effects of acrylamide in nontumorigenic breast cells by assessing expression levels of inducible nitric oxide synthase (iNOS) and cycloogenase-2 (Cox-2) and NOS activity, which are known to be early molecular changes in disease formation. Treatment of cells with acrylamide increased levels of iNOS (both expression and activity) and Cox-2. Its potent metabolite, glycidamide, also induced both iNOS and Cox-2, with induction of iNOS occurring at a lower concentration. 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), another food-borne carcinogen, was found to induce Cox-2 expression. Combining acrylamide with PhIP did not result in a further increase. These studies suggest that further research is needed to determine the role of carcinogens formed from cooking foods in inducing early molecular changes associated with breast cancer.Keywords
This publication has 42 references indexed in Scilit:
- Expression profile of human cells in culture exposed to glycidamide, a reactive metabolite of the heat-induced food carcinogen acrylamideToxicology, 2007
- Comparison of Estimated Dietary Intake of Acrylamide with Hemoglobin Adducts of Acrylamide and GlycidamideToxicological Sciences, 2007
- Excretion of mercapturic acids of acrylamide and glycidamide in human urine after single oral administration of deuterium-labelled acrylamideArchives of Toxicology, 2005
- Human exposure and internal dose assessments of acrylamide in foodFood and Chemical Toxicology, 2005
- Acrylamide and glycidamide: genotoxic effects in V79-cells and human bloodMutation Research/Genetic Toxicology and Environmental Mutagenesis, 2005
- A Mechanistic Basis for the Role of Cycle Arrest in the Genetic Toxicology of the Dietary Carcinogen 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)Toxicological Sciences, 2005
- Inducible nitric oxide synthase expression in N‐nitrosomethylbenzylamine (NMBA)‐induced rat esophageal tumorigenesisMolecular Carcinogenesis, 2004
- Epidemiologic evidence for assessing the carcinogenicity of acrylamideRegulatory Toxicology and Pharmacology, 2004
- COX-2 expression is associated with an aggressive phenotype in ductal carcinoma in situBritish Journal of Cancer, 2004
- Risk Assessment of Acrylamide in FoodsToxicological Sciences, 2003