Intramolecular disulfide bonding is essential for betanodavirus coat protein conformation
- 1 September 2002
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 83 (9), 2211-2214
- https://doi.org/10.1099/0022-1317-83-9-2211
Abstract
Here we report on the conformational changes that are responsible for the appearance of theDicentrarchus labraxencephalitis virus (DlEV) coat protein as a doublet in SDS–PAGE. Wild-type and mutated forms of the coat protein cDNA were expressed inE. coli. The study of the resulting recombinant molecules excluded the possibility of the involvement of a precursor autocatalysis mechanism or a ribosomal frameshifting event in the doublet formation. The appearance of the coat protein doublet was found to be β-mercaptoethanol sensitive. Based on this observation, we carried out substitution of all cysteine residues. The obtained results demonstrated the importance of intramolecular disulfide bonding between cysteines 187 and 201 on coat protein conformational changes.Keywords
This publication has 22 references indexed in Scilit:
- A fish encephalitis virus that differs from other nodaviruses by its capsid protein processingArchiv für die gesamte Virusforschung, 1997
- Viral nervous necrosis (VNN) associated with mass mortalities in cage‐reared sea bass,Dicentrarchus labrax (L.)Journal of Fish Diseases, 1997
- RECODING: Dynamic Reprogramming of TranslationAnnual Review of Biochemistry, 1996
- Ribosomal frameshifting on viral RNAsJournal of General Virology, 1995
- Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fishJournal of General Virology, 1995
- Folding and Assembly of Viral Membrane ProteinsVirology, 1993
- Structural homology among four nodaviruses as deduced by sequencing and X-ray crystallographyJournal of Molecular Biology, 1990
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Absence of Poly (A) from the Infective RNA of Nodamura VirusJournal of General Virology, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970