Assessment of the roles of various inactivation agents in an argon-based direct current atmospheric pressure cold plasma jet
- 15 June 2012
- journal article
- research article
- Published by AIP Publishing in Journal of Applied Physics
- Vol. 111 (12), 123305
- https://doi.org/10.1063/1.4730627
Abstract
Three types of gases, pure argon (99.999%), argon with 2% oxygen, and argon with 2% oxygen and 10% nitrogen were used as operating gases of a direct current atmospheric pressure cold plasma jet to inactivate Staphylococcus aureus (S. aureus) suspended in a liquid. The inactivation efficacies for the plasma jets operating in the three gases decrease from Ar/O2(2%) to Ar/O2(2%)/N2(10%) to pure Ar. Optical emission spectroscopy, electron spin resonance spectroscopy, high performance liquid chromatography, and atomic absorption spectrophotometry were employed to identify and monitor the reactive species in the plasma-liquid system for the three operating gases and revealed the presence of O, 1O2, OH, NO, H2O2, O3, and NO3−/NO2− as well as Cu+/Cu2+. The S. aureus inactivation results indicate that atomic oxygen (O) is the key inactivation agent, while other species play a lesser role in the inactivation progress studied here.Keywords
This publication has 31 references indexed in Scilit:
- Low-pressure water vapour plasma treatment of surfaces for biomolecules decontaminationJournal of Physics D: Applied Physics, 2012
- Reactive Oxygen Species in a Non‐thermal Plasma Microjet and Water System: Generation, Conversion, and Contributions to Bacteria Inactivation—An Analysis by Electron Spin Resonance SpectroscopyPlasma Processes and Polymers, 2012
- Inactivation of Bacillus subtilis Spores in Water by a Direct‐Current, Cold Atmospheric‐Pressure Air Plasma MicrojetPlasma Processes and Polymers, 2011
- Influence of the Air Humidity on the Reduction of Bacillus Spores in a Defined Environment at Atmospheric Pressure Using a Dielectric Barrier Surface DischargePlasma Processes and Polymers, 2010
- Bacterial inactivation using atmospheric pressure single pin electrode microplasma jet with a ground ringApplied Physics Letters, 2009
- Sterilization and decontamination of medical instruments by low-pressure plasma discharges: application of Ar/O2/N2ternary mixtureJournal of Physics D: Applied Physics, 2009
- Reactive Species and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic LifePlant Physiology, 2006
- Physics of high-pressure helium and argon radio-frequency plasmasJournal of Applied Physics, 2004
- Nitric oxide production and endothelium‐dependent vasorelaxation induced by wine polyphenols in rat aortaBritish Journal of Pharmacology, 1997
- Studies of atomic oxygen in O2+CF4 rf discharges by two-photon laser-induced fluorescence and optical emission spectroscopyThe Journal of Chemical Physics, 1986