Chromatographic determination method for 1‐nitropyrene and its metabolites in biological samples with fluorescence detection after on‐line reduction
- 1 September 1993
- journal article
- review article
- Published by Wiley in Biomedical Chromatography
- Vol. 7 (5), 262-266
- https://doi.org/10.1002/bmc.1130070505
Abstract
A high performance liquid chromatographic (HPLC) method with fluorescence detection after on-line reduction for the determination of 1-nitropyrene (NP), 1-nitrosopyrene (NSP), 1-aminopyrene (AP) and N-acetylaminopyrene (AAP) has been developed. The reduction efficiency of NP and NSP on a zinc column was found to be higher than that of an electrochemical reducer. Using a HPLC equipped with a zinc column (4.0 mm i.d. × 10 mm) and an imidazole/HCIO4 (pH 6.8):acetonitrile mobile phase, detection limits (S/N = 3) of 20-30 fmol for NP, NSP and AP and 350 fmol for AAP were obtained. NP, NSP and AP were determined in the incubation mixture of NP and Salmonella typhimurium, YG1021, by this method. Time course studies showed that a large ratio of NP was metabolized in the pre-incubation step of the Ames test.Keywords
This publication has 8 references indexed in Scilit:
- High-performance liquid chromatography retention index and detection of nitrated polycyclic aromatic hydrocarbonsJournal of Chromatography A, 1991
- Determination of nitrated pyrenes and their derivatives by high performance liquid chromatography with chemiluminescence detection after online electrochemical reductionBiomedical Chromatography, 1990
- Determination of nitro-polynuclear aromatic hydrocarbons in air and diesel particulate matter using liquid chromatography with electrochemical and fluorescence detectionAnalytical Chemistry, 1988
- Fluorescence detection and identification of nitro derivatives of polynuclear aromatic hydrocarbons by on-column catalytic reduction to aromatic aminesAnalytical Chemistry, 1986
- 1-nitrosopyrene: An intermediate in the metabolic activation of 1-nitropyrene to a mutagen in Salmonella typhimurium TA1538☆Mutation Research, 1985
- Detection of nitro-polycyclic aromatic hydrocarbons in liquid chromatography by zinc reduction and peroxyoxalate chemiluminescenceJournal of Chromatography A, 1984
- Reduction of the carcinogen 1-nitropyrene to 1-aminopyrene by rat intestinal bacteriaCarcinogenesis: Integrative Cancer Research, 1983
- Metabolism of 1-nitropyrene and formation of DNA adducts in Salmonella typhimuriumCarcinogenesis: Integrative Cancer Research, 1981