Label-Free Quantitative Mass Spectrometry Reveals a Panel of Differentially Expressed Proteins in Colorectal Cancer
Open Access
- 28 January 2015
- journal article
- review article
- Published by Hindawi Limited in BioMed Research International
- Vol. 2015, 1-13
- https://doi.org/10.1155/2015/365068
Abstract
To identify potential biomarkers involved in CRC, a shotgun proteomic method was applied to identify soluble proteins in three CRCs and matched normal mucosal tissues using high-performance liquid chromatography and mass spectrometry. Label-free protein profiling of three CRCs and matched normal mucosal tissues were then conducted to quantify and compare proteins. Results showed that 67 of the 784 identified proteins were linked to CRC (28 upregulated and 39 downregulated). Gene Ontology and DAVID databases were searched to identify the location and function of differential proteins that were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, and so on. Among the differentially expressed proteins, tropomyosin-3 (TPM3), endoplasmic reticulum resident protein 29 (ERp29), 18 kDa cationic antimicrobial protein (CAMP), and heat shock 70 kDa protein 8 (HSPA8) were verified to be upregulated in CRC tissue and seven cell lines through western blot analysis. Furthermore, the upregulation of TPM3, ERp29, CAMP, and HSPA8 was validated in 69 CRCs byimmunohistochemistry (IHC) analysis. Combination of TPM3, ERp29, CAMP, and HSPA8 can identify CRC from matched normal mucosal achieving an accuracy of 73.2% using IHC score. These results suggest that TPM3, ERp29, CAMP, and HSPA8 are great potential IHC diagnostic biomarkers for CRC.Funding Information
- China Postdoctoral Science Foundation (2013M532176)
This publication has 63 references indexed in Scilit:
- Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathwaysProceedings of the National Academy of Sciences of the United States of America, 2013
- The STRING database in 2011: functional interaction networks of proteins, globally integrated and scoredNucleic Acids Research, 2010
- Tropomyosin3 overexpression and a potential link to epithelial-mesenchymal transition in human hepatocellular carcinomaBMC Cancer, 2010
- Annual report to the nation on the status of cancer, 1975‐2006, featuring colorectal cancer trends and impact of interventions (risk factors, screening, and treatment) to reduce future ratesCancer, 2009
- Tropomyosin Isoform Expression Regulates the Transition of Adhesions To Determine Cell Speed and DirectionMolecular and Cellular Biology, 2009
- Proteomic Contributions to Personalized Cancer CareMolecular & Cellular Proteomics, 2008
- Proteomic Expression Analysis of Surgical Human Colorectal Cancer Tissues: Up-Regulation of PSB7, PRDX1, and SRP9 and Hypoxic Adaptation in CancerJournal of Proteome Research, 2008
- The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functionsFEBS Letters, 2007
- Contribution of oncoproteomics to cancer biomarker discoveryMolecular Cancer, 2007
- Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database SearchAnalytical Chemistry, 2002