In vivo self-bio-imaging of tumors through in situ biosynthesized fluorescent gold nanoclusters
Open Access
- 29 January 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), 1157
- https://doi.org/10.1038/srep01157
Abstract
Fluorescence imaging in vivo allows non-invasive tumor diagnostic thus permitting a direct monitoring of cancer therapies progresses. It is established herein that fluorescent gold nanoclusters are spontaneously biosynthesized by cancerous cell (i.e., HepG2, human hepatocarcinoma cell line; K562, leukemia cell line) incubated with micromolar chloroauric acid solutions, a biocompatible molecular Au(III) species. Gold nanoparticles form by Au(III) reduction inside cells cytoplasms and ultimately concentrate around their nucleoli, thus affording precise cell imaging. Importantly, this does not occur in non-cancerous cells, as evidenced with human embryo liver cells (L02) used as controls. This dichotomy is exploited for a new strategy for in vivo self-bio-imaging of tumors. Subcutaneous injections of millimolar chloroauric acid solution near xenograft tumors of the nude mouse model of hepatocellular carcinoma or chronic myeloid leukemia led to efficient biosynthesis of fluorescent gold nanoclusters without significant dissemination to the surrounding normal tissues, hence allowing specific fluorescent self-bio-marking of the tumors.This publication has 44 references indexed in Scilit:
- Cancer cells that survive radiation therapy acquire HIF-1 activity and translocate towards tumour blood vesselsNature Communications, 2012
- A bimetallic nanoantenna for directional colour routingNature Communications, 2011
- Cell-surface sensors for real-time probing of cellular environmentsNature Nanotechnology, 2011
- Optical imaging-guided cancer therapy with fluorescent nanoparticlesJournal of The Royal Society Interface, 2009
- From endoplasmic-reticulum stress to the inflammatory responseNature, 2008
- Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extractMaterials Letters, 2008
- Imaging in the era of molecular oncologyNature, 2008
- Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystalsBiomaterials, 2008
- Raman spectroscopy of DNA modified by intrastrand cross-links of antitumor cisplatinJournal of Structural Biology, 2007
- Surface Manipulation of the Electronic Energy of Subnanometer-Sized Gold Clusters: An Electrochemical and Spectroscopic InvestigationNano Letters, 2002