Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium,Synechococcus sp., and its transformation

Abstract

Among forty strains of marine cyanobacteria isolated in our laboratory, five strains had 1–3 different plasmids. The unicellular marine cyanobacterium,Synechococcus sp. NKBG 042902, contains at least three plasmids (pSY09, pSY10, and pSY11). However, these plasmids are cryptic. Therefore, a hybrid plasmid pUSY02 containing the 1.4kb HindIII fragment of pSY11 andEscherichia coli plasmid pUC18 was constructed. The plasmid pUSY02 transformed both marineSynechococcus sp. NKBG042902-YG1116, which is a cured strain, and fresh waterAnacystis nidulans R2 by dark incubation or Ca²⁺ treatment. However, the plasmid pSG111 constructed from the plasmid DNA ofA. nidulans R2 failed to transform marineSynechococcus sp. Electroporation was also applicable to transformation of marineSynechococcus sp. and fresh waterA. nidulans R2. The plasmid pUSY02 was rapidly introduced into marineSynechococcus sp.