Prevalence of the plasmid-mediated quinolone resistance determinants among clinical isolates of Shigella sp. in Andaman & Nicobar Islands, India
- 27 June 2011
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 53 (2), 247-251
- https://doi.org/10.1111/j.1472-765x.2011.03092.x
Abstract
This study was carried out to find the prevalence of various plasmid-mediated quinolone-resistant (PMQR) determinants among the quinolone-resistant clinical isolates of Shigella sp. from paediatric patients in Andaman & Nicobar Islands. A total of 106 quinolone-resistant Shigella isolates obtained from paediatric patients during hospital-based surveillance from January 2003 to June 2010 were screened for the presence of various PMQR determinants. Of 106 isolates, 8 (7.5%) showed the presence of aac (6')-Ib-cr and 3 (2.8%) harboured the qnrB genes with 2 (1.9%) of these isolates showing the presence of both. All the 9 isolates had uniform mutations in gyrA (S83L) and in parC (S80I). The prevalence of fluoroquinolone-acetylating aminoglycoside acetyltransferase {aac (6')-Ib-cr} gene is higher than qnrB gene among the clinical Shigella isolates. These PMQR determinants were detected in the Shigella isolates obtained from 2008-2010, indicating that it happens in a stepwise manner following the multiple mutations in quinolone resistance-determining regions increase or extend resistance to quinolones or fluoroquinolones. The prevalence of these genes are of grave concern as it may be horizontally transferred to other human pathogenic bacteria and can lead to therapeutic failure as a consequence of antimicrobial resistance, not only for the islands but also for the entire south-east region. The results obtained should encourage further studies on the implications of the presence, distribution, association and variation of these determinants in our quest for understanding PMQR.Keywords
This publication has 15 references indexed in Scilit:
- Prevalence of Plasmid-Mediated Quinolone Resistance Determinants over a 9-Year PeriodAntimicrobial Agents and Chemotherapy, 2009
- qnr Gene NomenclatureAntimicrobial Agents and Chemotherapy, 2008
- Analysis of the mechanisms of fluoroquinolone resistance in urinary tract pathogensJournal of Antimicrobial Chemotherapy, 2006
- qnrB , Another Plasmid-Mediated Gene for Quinolone ResistanceAntimicrobial Agents and Chemotherapy, 2006
- Emergence of plasmid-mediated resistance to quinolones in EnterobacteriaceaeJournal of Antimicrobial Chemotherapy, 2005
- Alteration in the GyrA Subunit of DNA Gyrase and the ParC Subunit of Topoisomerase IV in Quinolone-Resistant Shigella dysenteriae Serotype 1 Clinical Isolates from Kolkata, IndiaAntimicrobial Agents and Chemotherapy, 2005
- Cloning of a Novel Gene for Quinolone Resistance from a Transferable Plasmid in Shigella flexneri 2bAntimicrobial Agents and Chemotherapy, 2005
- Emergence of Nalidixic Acid-Resistant Shigella sonnei in Acute-Diarrhea Patients on Andaman and Nicobar Islands, IndiaAntimicrobial Agents and Chemotherapy, 2003
- Shigella infections among children in Andaman – an archipelago of tropical islands in Bay of BengalEpidemiology and Infection, 1998
- Quinolone resistance from a transferable plasmidThe Lancet, 1998