Histochemical and Immunohistochemical Study in Melasma: Evidence of Damage in the Basal Membrane
- 1 May 2011
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in The American Journal of Dermatopathology
- Vol. 33 (3), 291-295
- https://doi.org/10.1097/dad.0b013e3181ef2d45
Abstract
The pathogenesis of melasma has not been clearly elucidated. Using Fontana Masson; diastase-resistant periodic acid-Schiff stains; and immunohistochemistry to stem cell factor (SCF), its receptor c-kit, anti-mast cell tryptase, and anti-collagen type IV antibody, we evaluated melasma lesions and compared them with perilesional skin and photoprotected skin. Samples were taken from lesional and photoprotected nonlesional skin in 24 patients. In other 24 patients, we took biopsies of lesional and perilesional skin. With Fontana Masson, we observed many pigmented basal cells protruding into the dermis of the melasma skin. Periodic acid-Schiff stain and anti-collagen type IV showed damage on the basal membrane in 95.5% and 83%, respectively, in melasma lesion. The immunoreactivity of SCF and the prevalence of mast cells were increased in the dermis of melasma compared with perilesional dermis. The expression of c-kit was significantly increased at lesional epidermis; a frequent protrusion of c-kit-positive basal cells into the dermis was evident in 70% versus that in 29% of perilesional skin. The expression of c-kit was increased at lesional dermis of melasma compared with perilesional skin. We found a low correlation between c-kit expression and prevalence of mast cells; these were increased in melasma skin. The results may suggest a role of SCF, c-kit, and mast cells in the pathogenesis of melasma. We were surprised by the unexpected evidence of damage to basal membrane (BM), which could facilitate the fall or the migration of active melanocytes and melanin into the dermis allowing the constant hyperpigmentation in melasma.Keywords
This publication has 8 references indexed in Scilit:
- Oestrogen and progesterone receptor expression in melasma: an immunohistochemical analysisJournal of the European Academy of Dermatology and Venereology, 2010
- Solar elastosis and presence of mast cells as key features in the pathogenesis of melasmaClinical and Experimental Dermatology, 2008
- The vascular characteristics of melasmaJournal of Dermatological Science, 2007
- The dermal stem cell factor and c-kit are overexpressed in melasmaBritish Journal of Dermatology, 2006
- Two Cases of Melasma with Unusual Histopathologic FindingsJournal of Korean Medical Science, 2006
- Possible Involvement of Gelatinases in Basement Membrane Damage and Wrinkle Formation in Chronically Ultraviolet B-exposed Hairless MouseJournal of Investigative Dermatology, 2003
- Melasma: A clinical, light microscopic, ultrastructural, and immunofluorescence studyJournal of the American Academy of Dermatology, 1981
- Café au lait spots in Ataxia-telangiectasia (A.T.)Archiv für dermatologische Forschung, 1980