Cell death of barley aleurone protoplasts is mediated by reactive oxygen species
- 1 January 2001
- journal article
- Published by Wiley in The Plant Journal
- Vol. 25 (1), 19-29
- https://doi.org/10.1046/j.1365-313x.2001.00930.x
Abstract
The barley aleurone layer is a terminally differentiated secretory tissue whose activity is hormonally controlled. The plant hormone gibberellic acid (GA) stimulates the secretion of hydrolytic enzymes and triggers the onset of programmed cell death (PCD). Abscisic acid (ABA) antagonizes the effects of GA and inhibits enzyme secretion and PCD. Reactive oxygen species (ROS) are key players in many types of PCD, and data presented here implicate ROS in hormonally regulated death of barley aleurone cells. Incubation of aleurone layers or protoplasts in H(2)O(2)-containing media results in death of GA-treated but not ABA-treated aleurone cells. Cells that are programmed to die are therefore less able to withstand ROS than cells that are programmed to remain alive. Illumination of barley aleurone protoplasts with blue or UV-A light results in a rapid increase in intracellular H(2)O(2) production. GA-treated protoplasts die rapidly in response to this increase in intracellular H(2)O(2) production, but ABA-treated protoplasts do not die. The rate of light-induced death could be slowed by antioxidants, and incubating protoplasts in the dark with the antioxidant butylated hydroxy toluene reduces the rate of hormonally induced death. Taken together, these data demonstrate that GA-treated aleurone protoplasts are less able than ABA-treated protoplasts to tolerate internally generated or exogenously applied H(2)O(2), and strongly suggest that ROS are components of the hormonally regulated cell death pathway in barley aleurone cells.Keywords
This publication has 11 references indexed in Scilit:
- Riboswitch control of Rho-dependent transcription terminationProceedings of the National Academy of Sciences of the United States of America, 2012
- Whole-genome phylogenies of the family Bacillaceae and expansion of the sigma factor gene family in the Bacillus cereus species-groupBMC Genomics, 2011
- Bacillus anthracis genome organization in light of whole transcriptome sequencingBMC Bioinformatics, 2010
- Structure and Complexity of a Bacterial TranscriptomeJournal of Bacteriology, 2009
- Rapid, accurate, computational discovery of Rho-independent transcription terminators illuminates their relationship to DNA uptakeGenome Biology, 2007
- Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus groupInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Contribution of individual promoters in the ddlB–ftsZ region to the transcription of the essential cell‐division gene ftsZ in Escherichia coliMolecular Microbiology, 1997
- Transcription termination at intrinsic terminators: the role of the RNA hairpin.Proceedings of the National Academy of Sciences of the United States of America, 1995
- Developmental regulation of transcription of the Bacillus subtilis ftsAZ operonJournal of Molecular Biology, 1992
- SOME PHYSIOLOGICAL PROPERTIES OF DEXTRAL AND OF SINISTRAL FORMS IN BACILLUS MYCOIDES FLÜGGEThe Biological Bulletin, 1939