Serum‐free culture of PC13 murine embryonal carcinoma cells
- 1 June 1983
- journal article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 115 (3), 225-230
- https://doi.org/10.1002/jcp.1041150302
Abstract
The requirements for the serum‐free culture of PC13 murine embryonal carcinoma cells were determined. Supplementation of a 50:50 mixture of Dulbecco's modified Eagles medium and MCDB104 with transferrin (5 μg/ml), human high‐density lipoprotein (HDL) (100 μg/ml), and human low‐density lipoprotein (LDL) (50 μg/ml) supported growth comparable to that observed with 5% foetal calf serum. Media supplementation with lipoproteins apparently substitutes for the effects of insulin, desoctapeptide insulin (DOP), or multiplication‐stimulating activity (MSA) on EC cell multiplication. Clonal growth of PC13 EC cells in this serum‐free medium could only be achieved in the presence of suitable feeder cell monolayers. These observations demonstrate that PC13 EC cells do not have an absolute requirement for exogenous mitogens to support multiplication.Keywords
This publication has 25 references indexed in Scilit:
- Two multipotent embryonal carcinoma cell lines irreversibly differentiate in defined mediaDevelopmental Biology, 1983
- The location and synthesis of transferrin in mouse embryos and teratocarcinoma cellsDevelopmental Biology, 1982
- Insulin promotes the growth of F9 embryonal carcinoma cells apparently by acting through its own receptorBiochemical and Biophysical Research Communications, 1982
- Appearance of functional insulin receptors during the differentiation of embryonal carcinoma cells.The Journal of cell biology, 1981
- Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin, and dexamethasoneExperimental Cell Research, 1981
- High molecular weight extracellular proteins synthesized by endoderm cells derived from mouse teratocarcinoma cells and normal extraembryonic membranesDevelopmental Biology, 1980
- The differentiation of teratocarcinoma stem cells is marked by the types of collagen which are synthesizedCell, 1979
- Expression of the cell surface-associated glycoprotein, fibronectin, in the early mouse embryoDevelopmental Biology, 1979
- Transforming viruses directly reduce the cellular growth requirement for a platelet derived growth factorJournal of Cellular Physiology, 1978
- Multiplication‐stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptidesJournal of Cellular Physiology, 1973