Neutrophils are significant producers of IL-10 during sepsis
- 1 February 2010
- journal article
- research article
- Published by Elsevier BV in Biochemical and Biophysical Research Communications
- Vol. 393 (1), 28-31
- https://doi.org/10.1016/j.bbrc.2010.01.066
Abstract
Sepsis is a syndrome involving systemic inflammation as well as an infectious focus. Accordingly, the host immune response to sepsis involves complex leukocyte interplay that is incompletely understood. It is known that the immunoregulatory cytokine, IL-10, is rapidly expressed during the early stages of sepsis. In a murine model of sepsis, we sought to elucidate which leukocytes are early IL-10 producers. Using a novel IL-10 transcriptional reporter mouse, we observed that splenic leukocytes produced little IL-10. At the site of infection, peritoneal neutrophils produced the highest levels of IL-10 among leukocytes. Using cytokine antibody labeling, we further show that peritoneal neutrophils had high amounts of intracellular IL-10. We next depleted neutrophils and found a 40% decrease in peritoneal IL-10 levels. Altogether, this report demonstrates that among leukocytes, neutrophils are significant contributors of IL-10 at the site of infection during sepsis.Keywords
Funding Information
- National Institute of General Medical Sciences
This publication has 23 references indexed in Scilit:
- Selective Down-Regulation of Neutrophil Mac-1 in Endotoxemic Hepatic Microcirculation via IL-10Published by The American Association of Immunologists ,2009
- Nonredundant Roles for B Cell-Derived IL-10 in Immune Counter-RegulationPublished by The American Association of Immunologists ,2009
- γδ T cells mitigate the organ injury and mortality of sepsisJournal of Leukocyte Biology, 2007
- The disconnect between animal models of sepsis and human sepsisJournal of Leukocyte Biology, 2006
- THERMAL INJURY-INDUCED PRIMING EFFECT OF NEUTROPHIL IS TNF-α AND P38 DEPENDENTShock, 2006
- Anti‐inflammatory effect of interleukin‐10 on human neutrophil respiratory burst involves inhibition of GM‐CSF‐induced p47PHOXphosphorylation through a decrease in ERK1/2 activityThe FASEB Journal, 2006
- Interleukin-10 suppresses natural killer cell but not natural killer T cell activation during bacterial infectionCytokine, 2006
- An important regulatory role for CD4+CD8αα T cells in the intestinal epithelial layer in the prevention of inflammatory bowel diseaseProceedings of the National Academy of Sciences of the United States of America, 2003
- Interleukin-10 and the Interleukin-10 ReceptorAnnual Review of Immunology, 2001
- Interferon-gamma and interleukin-10 messenger RNA are up-regulated after orthotopic liver transplantation in tolerant rats: Evidence for cytokine-mediated immune dysregulationSurgery, 1995