In Vitro Organogenesis of Liver Tissuea
- 17 December 1997
- journal article
- review article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 831 (1), 382-397
- https://doi.org/10.1111/j.1749-6632.1997.tb52212.x
Abstract
The high metabolic rate of hepatocytes severely limits the mass of cells which can be transplanted without a vascular supply. We are developing an alternative approach in which vascularized tissue is grown ex vivo for anastamosis into the portal vein. Here, we discuss the key design issues for in vitro organogenesis of vascularized hepatic tissue, describe a fabrication approach for making complex degradable polymer scaffolds to organize cells in three dimensions on the scale of hundreds of microns, and demonstrate the feasibility of using these scaffolds for in vitro tissue organization in mixed-cell cultures.Keywords
This publication has 15 references indexed in Scilit:
- Solid free-form fabrication of drug delivery devicesJournal of Controlled Release, 1996
- In vitro cell response to differences in poly-L-lactide crystallinityJournal of Biomedical Materials Research, 1996
- Fulminant hepatic failure: Summary of a workshopHepatology, 1995
- Successful ex vivo gene therapy directed to liver in a patient with familial hypercholesterolaemiaNature Genetics, 1994
- Isolated hepatocytes in a bioartificial liver: A single group view and experienceBiotechnology & Bioengineering, 1994
- Three Dimensional Printing: Rapid Tooling and Prototypes Directly from a CAD ModelJournal of Engineering for Industry, 1992
- Hepatocyte culture on biodegradable polymeric substratesBiotechnology & Bioengineering, 1991
- Tissue Engineering by Cell Transplantation Using Degradable Polymer SubstratesJournal of Biomechanical Engineering, 1991
- Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities.The Journal of cell biology, 1985
- Reconstruction of Tissues by Dissociated CellsScience, 1963