Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential
- 17 December 2010
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 96A (2), 301-312
- https://doi.org/10.1002/jbm.a.32978
Abstract
We developed a method to produce discrete fibrin microthreads, which can be seeded with human mesenchymal stem cells (hMSCs) and used as a suture to enhance the efficiency and localization of cell delivery. To assess the efficacy of fibrin microthreads to support hMSC attachment, proliferation, and survival, microthreads (100 μm diameter per microthread) were bundled together, seeded with 50,000 hMSCs for 2 h, and cultured for 5 days. Cell density on microthread bundles increased over time in culture to a maximum average density of 731 ± 101 cells/mm2 after 5 days. A LIVE/DEAD assay confirmed that the cells were viable, and Ki-67 staining verified hMSC proliferation. In addition, functional differentiation assays demonstrated that hMSCs cultured on microthreads retained their ability to differentiate into adipocytes and osteocytes. The results of this study demonstrate that fibrin microthreads support hMSC viability and proliferation, while maintaining their multipotency. We anticipate that these cell-seeded fibrin microthreads will serve as a platform technology to improve localized delivery and engraftment of viable cells to damaged tissue. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.Keywords
This publication has 25 references indexed in Scilit:
- Adipogenesis in Nonadherent and Adherent Bone Marrow Stem Cells Grown in Fibrin Gel and in the Presence of Adult PlasmaCells Tissues Organs, 2007
- A Tissue Engineering Approach to Progenitor Cell Delivery Results in Significant Cell Engraftment and Improved Myocardial RemodelingThe International Journal of Cell Cloning, 2007
- Discrete crosslinked fibrin microthread scaffolds for tissue regenerationJournal of Biomedical Materials Research Part A, 2007
- Finding Fluorescent Needles in the Cardiac Haystack: Tracking Human Mesenchymal Stem Cells Labeled with Quantum Dots for Quantitative In Vivo Three-Dimensional Fluorescence AnalysisThe International Journal of Cell Cloning, 2007
- Cardiac RegenerationCirculation, 2006
- Artificial Matrix Helps Neonatal Cardiomyocytes Restore Injured Myocardium in RatsArtificial Organs, 2006
- Fibrin as a cell carrier in cardiovascular tissue engineering applicationsBiomaterials, 2005
- Mesenchymal stem cells: clinical applications and biological characterizationThe International Journal of Biochemistry & Cell Biology, 2004
- Fibrin Glue Alone and Skeletal Myoblasts in a Fibrin Scaffold Preserve Cardiac Function after Myocardial InfarctionTissue Engineering, 2004
- The Ki-67 protein: From the known and the unknownJournal of Cellular Physiology, 2000