Identification and validation of genes involved in cervical tumourigenesis
Open Access
- 22 February 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Cancer
- Vol. 11 (1), 80
- https://doi.org/10.1186/1471-2407-11-80
Abstract
Cervical cancer is the most common cancer among Indian women. This cancer has well defined pre-cancerous stages and evolves over 10-15 years or more. This study was undertaken to identify differentially expressed genes between normal, dysplastic and invasive cervical cancer.A total of 28 invasive cervical cancers, 4 CIN3/CIS, 4 CIN1/CIN2 and 5 Normal cervix samples were studied. We have used microarray technique followed by validation of the significant genes by relative quantitation using Taqman Low Density Array Real Time PCR. Immunohistochemistry was used to study the protein expression of MMP3, UBE2C and p16 in normal, dysplasia and cancers of the cervix. The effect of a dominant negative UBE2C on the growth of the SiHa cells was assessed using a MTT assay.Our study, for the first time, has identified 20 genes to be up-regulated and 14 down-regulated in cervical cancers and 5 up-regulated in CIN3. In addition, 26 genes identified by other studies, as to playing a role in cervical cancer, were also confirmed in our study. UBE2C, CCNB1, CCNB2, PLOD2, NUP210, MELK, CDC20 genes were overexpressed in tumours and in CIN3/CIS relative to both Normal and CIN1/CIN2, suggesting that they could have a role to play in the early phase of tumorigenesis. IL8, INDO, ISG15, ISG20, AGRN, DTXL, MMP1, MMP3, CCL18, TOP2A AND STAT1 were found to be upregulated in tumours. Using Immunohistochemistry, we showed over-expression of MMP3, UBE2C and p16 in cancers compared to normal cervical epithelium and varying grades of dysplasia. A dominant negative UBE2C was found to produce growth inhibition in SiHa cells, which over-expresses UBE2C 4 fold more than HEK293 cells.Several novel genes were found to be differentially expressed in cervical cancer. MMP3, UBE2C and p16 protein overexpression in cervical cancers was confirmed by immunohistochemistry. These will need to be validated further in a larger series of samples. UBE2C could be evaluated further to assess its potential as a therapeutic target in cervical cancer.Keywords
This publication has 84 references indexed in Scilit:
- Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinomaEuropean Journal of Surgical Oncology, 2009
- Integrated genomic and transcriptional profiling identifies chromosomal loci with altered gene expression in cervical cancerGenes, Chromosomes and Cancer, 2008
- Transcriptomic dissection of tongue squamous cell carcinomaBMC Genomics, 2008
- Human papillomavirus type 16 reduces the expression of microRNA-218 in cervical carcinoma cellsOncogene, 2007
- Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomasInternational Journal of Cancer, 2007
- Comprehensive Expression Profiling of Tumor Cell Lines Identifies Molecular Signatures of Melanoma ProgressionPLOS ONE, 2007
- p53FamTaG: a database resource of human p53, p63 and p73 direct target genes combining in silico prediction and microarray dataBMC Bioinformatics, 2007
- Molecular profiling of cervical cancer progressionBritish Journal of Cancer, 2007
- BABELOMICS: a systems biology perspective in the functional annotation of genome-scale experimentsNucleic Acids Research, 2006
- Significance analysis of microarrays applied to the ionizing radiation responseProceedings of the National Academy of Sciences of the United States of America, 2001