Cytokine mRNA Expression Is Decreased in the Subplantar Muscle of Rat Paw Subjected to Carrageenan-Induced Inflammation after Low-Level Laser Therapy
- 1 February 2008
- journal article
- Published by Mary Ann Liebert Inc in Photomedicine and Laser Surgery
- Vol. 26 (1), 19-24
- https://doi.org/10.1089/pho.2007.2119
Abstract
The objective of this work was to investigate the anti-inflammatory effects of low-level laser therapy, applied at different wavelengths (660 and 684 nm), on cytokine mRNA expression after carrageenan-induced acute inflammation in rat paw. Low-level laser therapy (LLLT) has been observed to reduce pain in inflammatory disorders. However, little is known about the mechanisms behind this effect or whether it is wavelength-specific. The test sample consisted of 32 rats divided into four groups: A(1) (control-saline), A(2) (carrageenan-only), A(3) (carrageenan + 660 nm laser therapy), and A(4) (carrageenan + 684 nm laser therapy). The animals from groups A(3) and A(4) were irradiated 1 h after induction of inflammation by carrageenan injection. Continuous-wave red lasers with wavelengths of 660 and 684 nm and dose of 7.5 J/cm(2) were used. Both the 660 nm and 684 nm laser groups had 30%-40% lower mRNA expression for cytokines TNF-alpha, IL-1beta, and IL-6 in the paw muscle tissue than the carrageenan-only control group. Cytokine measurements were made 3 h after laser irradiation of the paw muscle, and all cytokine differences between the carrageenan-only control group and the LLLT groups were statistically significant (p < 0.001). LLLT at the 660-nm and 684-nm wavelengths administered to inflamed rat paw tissue at a dose of 7.5 J/cm(2) reduce cytokine mRNA expression levels within 3 h in the laser-irradiated tissue.Keywords
This publication has 33 references indexed in Scilit:
- Low-Level Laser Therapy in Acute Pain: A Systematic Review of Possible Mechanisms of Action and Clinical Effects in Randomized Placebo-Controlled TrialsPhotomedicine and Laser Surgery, 2006
- Cytokine networks—towards new therapies for rheumatoid arthritisNature Clinical Practice Rheumatology, 2005
- Immunomodulatory effects of low-intensity near-infrared laser irradiation on contact hypersensitivity reactionPhotodermatology, Photoimmunology & Photomedicine, 2003
- Cytokine stimulation of T lymphocytes regulates their capacity to induce monocyte production of tumor necrosis factor‐α, but not interleukin‐10: Possible relevance to pathophysiology of rheumatoid arthritisEuropean Journal of Immunology, 1997
- Interleukin-15 mediates T cell-dependent regulation of tumor necrosis factor-α production in rheumatoid arthritisNature Medicine, 1997
- ROLE OF CYTOKINES IN RHEUMATOID ARTHRITISAnnual Review of Immunology, 1996
- The role of interleukin–15 in T–cell migration and activation in rheumatoid arthritisNature Medicine, 1996
- Mast cells, cytokines, and metalloproteinases at the rheumatoid lesion: dual immunolocalisation studies.Annals Of The Rheumatic Diseases, 1995
- Inhibition of the production and effects of interleukins‐1 and tumor necrosis factor α in rheumatoid arthritisArthritis & Rheumatism, 1995
- DETECTION OF CYTOKINES AT THE CARTILAGE/PANNUS JUNCTION IN PATIENTS WITH RHEUMATOID ARTHRITIS: IMPLICATIONS FOR THE ROLE OF CYTOKINES IN CARTILAGE DESTRUCTION AND REPAIRRheumatology, 1992