Crystallization of the reovirus type 3 dearing core crystal packing is determined by the λ2 protein
- 5 September 1990
- journal article
- editorial
- Published by Elsevier BV in Journal of Molecular Biology
- Vol. 215 (1), 1-5
- https://doi.org/10.1016/s0022-2836(05)80089-0
Abstract
Core particles of reovirus type 3 Dearing (T3D) crystallized in the face-centered cubic space group F432 with dimensions of 1270 Å along each edge of the unit cell. Core particles of reovirus type 1 Lang (T1L) did not crystallize. Experiments with core particles derived from 27 different T1L × T3D reassortant viruses indicated that the L2 genome segment determined the capacity of cores to crystallize. This finding indicates important differences in the surface topography of the L2-translation product, the λ2 protein, of these two isolates, and suggests that important crystal contacts are mediated by this protein. These data are used to generate a model of the packing of reovirus core particles within the unit cell.Keywords
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