Prevalence and antibiogram of Extended Spectrum β-Lactamase (ESBL) producing Gram negative bacilli and further molecular characterization of ESBL producing Escherichia coli and Klebsiella spp.
Open Access
- 1 January 2013
- journal article
- Published by JCDR Research and Publications in Journal of Clinical and Diagnostic Research
- Vol. 7 (10), 2173-7
- https://doi.org/10.7860/jcdr/2013/6460.3462
Abstract
Resistance to a wide variety of common antimicrobials has made the proliferation of Extended spectrum β-lactmase (ESBL) producing strains a serious global health concern that has complicated treatment strategies. The high proportion of ESBL producers among the Enterobacteriaceae and the complex molecular epidemiology with diverse types of ESBL genes are alarming. This study was undertaken to identify ESBL production in various Gram negative bacilli isolated and to further characterize ESBL producers among Escherichia coli and Klebsiella spp by PCR, which were initially screened by phenotypic method. A total of 722 isolates of Gram negative bacilli were isolated. Presence of ESBL positivity was detected using the double disk synergy test (DDST). Their antibiogram was studied. PCR analysis for β-lactamase (bla) genes of the family TEM, SHV and CTX-M was also carried out using designed primers in 20 ESBL isolates each of Escherichia coli and Klebsiella spp. Among 722 Gram negative bacilli isolated 379 (52.49%) were ESBL producers. The major source of ESBL producers were respiratory tract samples, highest ESBL production was observed in Klebsiella sp. (67.04%). Resistance to multiple classes of antibiotics was observed among ESBL producers. Among ESBL producing genes prevalence of bla-CTX-M (82.5%) was highest, followed by bla-TEM (67.5%) and bla-SHV (57.5%) in the present study. The frequency of ESBL producing strains among clinical isolates has been steadily increasing. Advance drug resistance surveillance and molecular characteristics of ESBL isolates is necessary to guide the appropriate and judicious antibiotic use.Keywords
This publication has 25 references indexed in Scilit:
- Epidemiology and outcome of bacteremia caused by extended spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. in a tertiary care teaching hospital in south India.2010
- Changing trends in antimicrobial susceptibility and hospital acquired infections over an 8 year period in a tertiary care hospital in relation to introduction of an infection control programme.2010
- Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from hospitals in LithuaniaJournal of Medical Microbiology, 2010
- Molecular Characterization and Epidemiology of Extended-Spectrum- β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates Causing Health Care-Associated Infection in Thailand, Where the CTX-M Family Is EndemicAntimicrobial Agents and Chemotherapy, 2008
- The continuing challenge of ESBLsCurrent Opinion in Pharmacology, 2007
- Extended-Spectrum β-Lactamases: a Clinical UpdateClinical Microbiology Reviews, 2005
- Resistance to ?-lactam antibioticsCellular and Molecular Life Sciences, 2004
- Extended Spectrum ??-Lactamase (ESBL)-Producing EnterobacteriaceaeDrugs, 2003
- Extended‐Spectrum β‐Lactamases: Epidemiology, Detection, and TreatmentPharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 2001
- Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E testEuropean Journal of Clinical Microbiology & Infectious Diseases, 1996