The GO system prevents ROS-induced mutagenesis and killing inPseudomonas aeruginosa
Open Access
- 1 May 2009
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 294 (1), 89-96
- https://doi.org/10.1111/j.1574-6968.2009.01550.x
Abstract
Inactivation of the Pseudomonas aeruginosa mutM, mutY, or mutT gene conferred a 2.4-, 17.2-, or 38.1-fold increase in spontaneous mutation frequency, respectively. Importantly, the mutY and mutT strains each displayed a robust H2O2-induced mutation frequency. In addition, the mutM, mutY, and mutT mutations severely sensitized P. aeruginosa to killing by H2O2, suggesting that these gene products act to repair one or more cytotoxic lesions in P. aeruginosa. Nucleotide sequence analysis of a fragment of the rpoB gene from rifampicin resistant mutM-, mutY-, and, mutT-deficient strains was consistent with this conclusion. These findings are discussed in terms of possible roles for mutM, mutY, and mutT in contributing to survival and mutagenesis of P. aeruginosa colonizing the airways of cystic fibrosis patients.Keywords
This publication has 27 references indexed in Scilit:
- Role ofPseudomonas aeruginosa dinB-Encoded DNA Polymerase IV in MutagenesisJournal of Bacteriology, 2006
- Lack of Association between Hypermutation and Antibiotic Resistance Development inPseudomonas aeruginosaIsolates from Intensive Care Unit PatientsAntimicrobial Agents and Chemotherapy, 2004
- Comprehensive transposon mutant library of Pseudomonas aeruginosaProceedings of the National Academy of Sciences of the United States of America, 2003
- The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative LesionsJournal of Bacteriology, 2003
- Use of the rpoB gene to determine the specificity of base substitution mutations on the Escherichia coli chromosomeDNA Repair, 2003
- Characterization of the GO system ofPseudomonas aeruginosaFEMS Microbiology Letters, 2002
- Adaptive mutations, mutator DNA polymerases and genetic change strategies of pathogensCurrent Opinion in Microbiology, 2001
- Characterization of a Novel 8-Oxoguanine-DNA Glycosylase Activity in Escherichia coli and Identification of the Enzyme as Endonuclease VIIIOnline Journal of Public Health Informatics, 2000
- Recognition of Formamidopyrimidine by Escherichia coli and Mammalian Thymine Glycol GlycosylasesOnline Journal of Public Health Informatics, 2000
- High Frequency of Hypermutable Pseudomonas aeruginosa in Cystic Fibrosis Lung InfectionScience, 2000