Comparative Proteomics Analysis Suggests that Placental Mitochondria are Involved in the Development of Pre-Eclampsia
Open Access
- 9 May 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (5), e64351
- https://doi.org/10.1371/journal.pone.0064351
Abstract
INTRODUCTION: Pre-eclampsia (PE), a severe pregnancy-specific disease characterized by the new onset of hypertension, proteinuria, edema, and a series of other systematic disorders, is a state of widespread mitochondrial dysfunction of the placenta. METHODS: We compared the morphology of mitochondria in pre-eclamptic and normotensive placentae using electron microscopy. To reveal the systematic protein expression changes of placental mitochondria that might explain the pathogenesis of PE, we performed iTRAQ analysis combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on differentially expressed placental mitochondria proteins from 4 normotensive and 4 pre-eclamptic pregnancies. Bioinformatics analysis was used to find the relative processes that these differentially expressed proteins were involved in. Three differentially expressed proteins were chosen to confirm by Western blotting and immunohistochemistry. RESULTS: Morphological data demonstrated degenerative and apoptotic changes in the mitochondria of pre-eclamptic placentae. We found four proteins were upregulated and 22 proteins were downregulated in pre-eclamptic placentae compared with normotensive placentae. Bioinformatics analysis showed that these proteins were involved in many critical processes in the development of pre-eclampsia such as apoptosis, fatty acid oxidation, the respiratory chain, reactive oxygen species generation, the tricarboxylic acid cycle and oxidative stress. CONCLUSIONS: This preliminary work provides a better understanding of the proteomic alterations of mitochondria from pre-eclamptic placentae and may aid in our understanding of the importance of mitochondria in the development of pre-eclampsiaKeywords
This publication has 34 references indexed in Scilit:
- Comparative mitochondrial proteomics: perspective in human diseasesJournal of Hematology & Oncology, 2012
- Periostin identified as a potential biomarker of prostate cancer by iTRAQ-proteomics analysis of prostate biopsyProteome Science, 2011
- Placental Apoptosis in Health and DiseaseAmerican Journal of Reproductive Immunology, 2010
- Eight‐plex iTRAQ analysis of variant metastatic human prostate cancer cells identifies candidate biomarkers of progression: An exploratory studyThe Prostate, 2010
- iTRAQ-Facilitated Proteomic Analysis of Human Prostate Cancer Cells Identifies Proteins Associated with ProgressionJournal of Proteome Research, 2008
- Toward a better understanding of preeclampsia: Comparative proteomic analysis of preeclamptic placentasProteomics – Clinical Applications, 2007
- De Novo Biosynthetic Profiling of High Abundance Proteins in Cystic Fibrosis Lung Epithelial CellsMolecular & Cellular Proteomics, 2006
- Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging ReagentsMolecular & Cellular Proteomics, 2004
- Reactive oxygen species and mitochondrial diseasesSeminars in Cell & Developmental Biology, 2001
- Pre‐Eclampsia‐A Mitochondrial Disease?Acta Obstetricia et Gynecologica Scandinavica, 1989