Heterogeneity of Vaginal Microbial Communities within Individuals
- 1 April 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (4), 1181-1189
- https://doi.org/10.1128/jcm.00854-08
Abstract
Recent culture-independent studies have revealed that a healthy vaginal ecosystem harbors a surprisingly complex assemblage of microorganisms. However, the spatial distribution and composition of vaginal microbial populations have not been investigated using molecular methods. Here, we evaluated site-specific microbial composition within the vaginal ecosystem and examined the influence of sampling technique in detection of the vaginal microbiota. 16S rRNA gene clone libraries were prepared from samples obtained from different locations (cervix, fornix, outer vaginal canal) and by different methods (swabbing, scraping, lavaging) from the vaginal tracts of eight clinically healthy, asymptomatic women. The data reveal that the vaginal microbiota is not homogenous throughout the vaginal tract but differs significantly within an individual with regard to anatomical site and sampling method used. Thus, this study illuminates the complex structure of the vaginal ecosystem and calls for the consideration of microenvironments when sampling vaginal microbiota as a clinical predictor of vaginal health.Keywords
This publication has 35 references indexed in Scilit:
- Critical Evaluation of Two Primers Commonly Used for Amplification of Bacterial 16S rRNA GenesApplied and Environmental Microbiology, 2008
- The human microbiome: eliminating the biomedical/environmental dichotomy in microbial ecologyEnvironmental Microbiology, 2007
- The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public dataNucleic Acids Research, 2006
- Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosisAmerican Journal of Obstetrics and Gynecology, 2004
- Social and sexual risk factors for bacterial vaginosisSexually Transmitted Infections, 2004
- The Identification of VaginalLactobacillusSpecies and the Demographic and Microbiologic Characteristics of Women Colonized by These SpeciesThe Journal of Infectious Diseases, 1999
- The human vagina: normal flora considered as an in situ tissue-associated, adherent biofilm.Sexually Transmitted Infections, 1991
- The Folded Normal DistributionTechnometrics, 1961
- The Folded Normal DistributionTechnometrics, 1961
- An Ordination of the Upland Forest Communities of Southern WisconsinEcological Monographs, 1957