Cell Cycle Control of DNA Replication by a Homologue from Human Cells of the p34 cdc2 Protein Kinase
- 9 November 1990
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 250 (4982), 786-791
- https://doi.org/10.1126/science.2173140
Abstract
The regulation of DNA replication during the eukaryotic cell cycle was studied in a system where cell free replication of simian virus 40 (SV40) DNA was used as a model for chromosome replication. A factor, RF-S, was partially purified from human S phase cells based on its ability to activate DNA replication in extracts from G1 cells. RF-S contained a human homologue of the Schizosaccharomyces pombe p34cdc2 kinase, and this kinase was necessary for RF-S activity. The limiting step in activation of the p34 kinase at the G1 to S transition may be its association with a cyclin since addition of cyclin A to a G1 extract was sufficient to start DNA replication. These observations suggest that the role of p34cdc2 in controlling the start of DNA synthesis has been conserved in evolution.Keywords
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