Off‐line comprehensive two‐dimensional hydrophilic interaction×reversed phase liquid chromatographic analysis of green tea phenolics
- 1 March 2010
- journal article
- research article
- Published by Wiley in Journal of Separation Science
- Vol. 33 (6-7), 853-863
- https://doi.org/10.1002/jssc.200900673
Abstract
The separation of green tea phenolic compounds by comprehensive 2-D LC (LC×LC) has been studied. An off-line LC×LC system was designed based on hydrophilic interaction chromatography in the first dimension and gradient RP-LC using a C18 column in the second dimension, to separate compounds based on polarity and hydrophobicity, respectively. Green tea was found to contain a complex composition of low molecular weight phenolic compounds, identified by fluorescence, diode-array and negative ESI-MS detection systems. The LC×LC system was characterized by a very high practical peak capacity (>2100, in ∼22.5 h) due to the low degree of correlation (r2<0.2) between the selected separation mechanisms. The proposed methodology demonstrated its suitability for the analysis of various groups of phenolic compounds including proanthocyanidins, phenolic acids, flavonols and flavonol derivatives, all of which cannot be separated in a single analysis by conventional 1-D HPLC methods.Keywords
This publication has 54 references indexed in Scilit:
- Preclinical development of the green tea catechin, epigallocatechin gallate, as an HIV-1 therapyJournal of Allergy and Clinical Immunology, 2009
- Equation for Peak Capacity Estimation in Two-Dimensional Liquid ChromatographyAnalytical Chemistry, 2008
- Inhibition of HIV-1-gp120 Attachment to CD4 on Hematopoietic Stem Cell Derived-Langerhans Cells by the Green Tea Catechin, Epigallocatechin GallateJournal of Allergy and Clinical Immunology, 2008
- Tea polyphenols for health promotionLife Sciences, 2007
- HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescenceJournal of Food Composition and Analysis, 2007
- Epigallocatechin gallate, the main polyphenol in green tea, binds to the T-cell receptor, CD4: Potential for HIV-1 therapyJournal of Allergy and Clinical Immunology, 2006
- Green tea and its polyphenolic catechins: Medicinal uses in cancer and noncancer applicationsLife Sciences, 2006
- Seasonal Variations of Phenolic Compounds in Australia-Grown Tea (Camellia sinensis)Journal of Agricultural and Food Chemistry, 2005
- Isolation and Determination of Phenolic Compounds in Fruit‐Green TeaJournal of Liquid Chromatography & Related Technologies, 2004
- Efficiency of the extraction of catechins from green teaFood Chemistry, 1999