Effects of aging, body mass index, plasma lipid profiles, and smoking on human plasma N-glycans
Open Access
- 30 March 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 20 (8), 959-969
- https://doi.org/10.1093/glycob/cwq051
Abstract
Protein glycosylation affects nearly all molecular interactions at the cell surface and in the intercellular space. Many of the physiological variations which are part of homeostatic mechanisms influence glycosylation. However, a comprehensive overview of changes in glycosylation caused by aging and common lifestyle parameters is still lacking. After analyzing N-glycans in the plasma of 1914 individuals from the Croatian islands of Vis and Korčula, we performed a comprehensive analysis of the dependence of different glycosylation features (position of fucose, level of galactosylation, sialylation and branching) on aging, smoking, body fat and plasma lipid status. A number of statistically significant associations were observed. Glycosylation changes with aging were especially evident in females, mostly in association with the transition from pre-menopausal to post-menopausal age. Levels of core-fucosylated, non-galactosylated, digalactosylated and disialylated biantennary glycans were shown to be mainly age dependent, but the level of branching and higher levels of galactosylation were found to correlate with lipid status. For the majority of glycans which we analyzed, all examined parameters explained up to 5% of the variance. The only notable exception were non-galactosylated glycans where 20% of the variance was explained mostly by age and blood pressure. In general, only a small fraction of the variability in glycan levels observed in a population was explained by age and other measured parameters, indicating that even in the absence of a genetic template, glycan levels are mostly determined by genetic background and/or specific pathophysiological processes.Keywords
This publication has 40 references indexed in Scilit:
- New genetic loci implicated in fasting glucose homeostasis and their impact on type 2 diabetes riskNature Genetics, 2010
- A HUPO test sample study reveals common problems in mass spectrometry–based proteomicsNature Methods, 2009
- Loci influencing lipid levels and coronary heart disease risk in 16 European population cohortsNature Genetics, 2008
- Effects of genome-wide heterozygosity on a range of biomedically relevant human quantitative traitsHuman Molecular Genetics, 2007
- Estimating Human Inbreeding Coefficients: Comparison of Genealogical and Marker Heterozygosity ApproachesAnnals of Human Genetics, 2006
- Fucose: biosynthesis and biological function in mammalsGlycobiology, 2003
- Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipmentGlycobiology, 2001
- Oral estrogen treatment induces a decrease in expression of sialyl Lewis x on α1 glycoprotein in females and male-to-female transsexualsGlycobiology, 1996
- Body mass index as a measure of body fatness: age- and sex-specific prediction formulasBritish Journal of Nutrition, 1991
- Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 YearsBritish Journal of Nutrition, 1974