Toward surface quantification of liver fibrosis progression
Open Access
- 1 January 2010
- journal article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 15 (5), 056007-056007-11
- https://doi.org/10.1117/1.3490414
Abstract
Monitoring liver fibrosis progression by liver biopsy is important for certain treatment decisions, but repeated biopsy is invasive. We envision redefinition or elimination of liver biopsy with surface scanning of the liver with minimally invasive optical methods. This would be possible only if the information contained on or near liver surfaces accurately reflects the liver fibrosis progression in the liver interior. In our study, we acquired the second-harmonic generation and two-photon excitation fluorescence microscopy images of liver tissues from bile duct-ligated rat model of liver fibrosis. We extracted morphology-based features, such as total collagen, collagen in bile duct areas, bile duct proliferation, and areas occupied by remnant hepatocytes, and defined the capsule and subcapsular regions on the liver surface based on image analysis of features. We discovered a strong correlation between the liver fibrosis progression on the anterior surface and interior in both liver lobes, where biopsy is typically obtained. The posterior surface exhibits less correlation with the rest of the liver. Therefore, scanning the anterior liver surface would obtain similar information to that obtained from biopsy for monitoring liver fibrosis progression.Keywords
This publication has 59 references indexed in Scilit:
- Probing myosin structural conformation in vivo by second-harmonic generation microscopyProceedings of the National Academy of Sciences of the United States of America, 2010
- Diagnosis and Quantitation of FibrosisGastroenterology, 2008
- Recent advances in the pathogenesis and diagnosis of liver fibrosisThe Esophagus, 2008
- Assessment of Hepatic Fibrosis With Magnetic Resonance ElastographyClinical Gastroenterology and Hepatology, 2007
- Reproducibility of transient elastography in the evaluation of liver fibrosis in patients with chronic liver diseaseGut, 2007
- In situ multiphoton optical tomography of hair follicles in miceJournal of Biomedical Optics, 2007
- Second Harmonic Generation Confocal Microscopy of Collagen Type I from Rat Tendon CryosectionsBiophysical Journal, 2006
- Noninvasive prediction of fibrosis in patients with chronic hepatitis CJournal of Hepatology, 2003
- The role of laparoscopy in the diagnosis of cirrhosisGastrointestinal Endoscopy, 1996
- Percutaneous blind biopsy versus laparoscopy with guided biopsy in diagnosis of cirrhosisDigestive Diseases and Sciences, 1983