Investigation ofβ-lactam antibacterial drugs,β-lactamases, and penicillin-binding proteins with fluorescence polarization and anisotropy: a review
- 12 April 2016
- journal article
- review article
- Published by IOP Publishing in Methods and Applications in Fluorescence
- Vol. 4 (2), 024002
- https://doi.org/10.1088/2050-6120/4/2/024002
Abstract
This review covers the uses of fluorescence polarization and anisotropy for the investigation of bacterial penicillin binding proteins (PBPs), which are the targets of β-lactam antibacterial drugs (penicillins, cephalosporins, carbapenems, and monobactams), and of the β-lactamase enzymes that destroy these drugs and help to render bacterial pathogens resistant to them. Fluorescence polarization and anisotropy-based methods for quantitation of β-lactam drugs are also reviewed. A particular emphasis is on methods for quantitative measurement of the interactions of β-lactams and other inhibitors with PBPs and β-lactamases.Keywords
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