An updated two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies
- 1 January 1995
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 16 (1), 1977-1981
- https://doi.org/10.1002/elps.11501601324
Abstract
We have improved upon the reference two‐dimensional (2‐D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907–930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2‐D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high‐performance liquid chromatography (HPLC), were sequenced using a Perkin‐Elmer 477A sequenator. Additional spots were identified using specific antibodies.Keywords
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