In vivo RNAi: Today and Tomorrow
- 9 June 2010
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Perspectives in Biology
- Vol. 2 (8), a003640
- https://doi.org/10.1101/cshperspect.a003640
Abstract
RNA interference (RNAi) provides a powerful reverse genetics approach to analyze gene functions both in tissue culture and in vivo. Because of its widespread applicability and effectiveness it has become an essential part of the tool box kits of model organisms such as Caenorhabditis elegans, Drosophila, and the mouse. In addition, the use of RNAi in animals in which genetic tools are either poorly developed or nonexistent enables a myriad of fundamental questions to be asked. Here, we review the methods and applications of in vivo RNAi to characterize gene functions in model organisms and discuss their impact to the study of developmental as well as evolutionary questions. Further, we discuss the applications of RNAi technologies to crop improvement, pest control and RNAi therapeutics, thus providing an appreciation of the potential for phenomenal applications of RNAi to agriculture and medicine.Keywords
This publication has 91 references indexed in Scilit:
- Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAiNature, 2009
- A simplified miRNA-based gene silencing method for Drosophila melanogasterDevelopmental Biology, 2008
- Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenesNature Genetics, 2008
- A genome-wide transgenic RNAi library for conditional gene inactivation in DrosophilaNature, 2007
- Identification of Genes Needed for Regeneration, Stem Cell Function, and Tissue Homeostasis by Systematic Gene Perturbation in PlanariaDevelopmental Cell, 2005
- Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegansNature, 2005
- Genome sequence of the Brown Norway rat yields insights into mammalian evolutionNature, 2004
- Initial sequencing and comparative analysis of the mouse genomeNature, 2002
- The origin of pattern and polarity in the Drosophila embryoCell, 1992
- Mutations affecting segment number and polarity in DrosophilaNature, 1980