Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission
Top Cited Papers
Open Access
- 1 March 2013
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 24 (5), 659-667
- https://doi.org/10.1091/mbc.e12-10-0721
Abstract
Several mitochondrial outer membrane proteins—mitochondrial fission protein 1 (Fis1), mitochondrial fission factor (Mff), mitochondrial dynamics proteins of 49 and 51 kDa (MiD49 and MiD51, respectively)—have been proposed to promote mitochondrial fission by recruiting the GTPase dynamin-related protein 1 (Drp1), but fundamental issues remain concerning their function. A recent study supported such a role for Mff but not for Fis1. In addition, it is unclear whether MiD49 and MiD51 activate or inhibit fission, because their overexpression causes extensive mitochondrial elongation. It is also unknown whether these proteins can act in the absence of one another to mediate fission. Using Fis1-null, Mff-null, and Fis1/Mff-null cells, we show that both Fis1 and Mff have roles in mitochondrial fission. Moreover, immunofluorescence analysis of Drp1 suggests that Fis1 and Mff are important for the number and size of Drp1 puncta on mitochondria. Finally, we find that either MiD49 or MiD51 can mediate Drp1 recruitment and mitochondrial fission in the absence of Fis1 and Mff. These results demonstrate that multiple receptors can recruit Drp1 to mediate mitochondrial fission.Keywords
This publication has 28 references indexed in Scilit:
- Mitotic Phosphorylation of Dynamin-related GTPase Drp1 Participates in Mitochondrial FissionPublished by Elsevier BV ,2007
- A Role for Fis1 in Both Mitochondrial and Peroxisomal Fission in Mammalian CellsMolecular Biology of the Cell, 2005
- Regulation of mitochondrial fission and apoptosis by the mitochondrial outer membrane protein hFis1Journal of Cell Science, 2005
- The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondriaThe Journal of cell biology, 2005
- Roles of the Mammalian Mitochondrial Fission and Fusion Mediators Fis1, Drp1, and Opa1 in ApoptosisMolecular Biology of the Cell, 2004
- Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphologyJournal of Cell Science, 2004
- The WD-repeats of Net2p Interact with Dnm1p and Fis1p to Regulate Division of MitochondriaMolecular Biology of the Cell, 2003
- The Mitochondrial Protein hFis1 Regulates Mitochondrial Fission in Mammalian Cells through an Interaction with the Dynamin-Like Protein DLP1Molecular and Cellular Biology, 2003
- The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fissionThe Journal of cell biology, 2002
- Dnm1p Gtpase-Mediated Mitochondrial Fission Is a Multi-Step Process Requiring the Novel Integral Membrane Component Fis1pThe Journal of cell biology, 2000