α Helix-RNA Major Groove Recognition in an HIV-1 Rev Peptide-RRE RNA Complex
- 13 September 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 273 (5281), 1547-1551
- https://doi.org/10.1126/science.273.5281.1547
Abstract
The solution structure of a human immunodeficiency virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of the Rev response element (RRE) RNA was solved by nuclear magnetic resonance spectroscopy. The Rev peptide has an α-helical conformation and binds in the major groove of the RNA near a purine-rich internal loop. Several arginine side chains make base-specific contacts, and an asparagine residue contacts a G·A base pair. The phosphate backbone adjacent to a G·G base pair adopts an unusual structure that allows the peptide to access a widened major groove. The structure formed by the two purine-purine base pairs of the RRE creates a distinctive binding pocket that the peptide can use for specific recognition.Keywords
This publication has 21 references indexed in Scilit:
- Selection of RNA-binding peptides in vivoNature, 1996
- Binding of an HIV Rev peptide to Rev responsive element RNA induces formation of purine-purine base pairsBiochemistry, 1994
- Helix stop signals in proteins and peptides: The capping boxBiochemistry, 1993
- Capping interactions in isolated .alpha. helixes: position-dependent substitution effects and structure of a serine-capped peptide helixBiochemistry, 1993
- The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted α Helices: Crystal structure of the protein-DNA complexCell, 1992
- Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experimentsFEBS Letters, 1992
- An efficient triple resonance experiment using carbon-13 isotropic mixing for determining sequence-specific resonance assignments of isotopically-enriched proteinsJournal of the American Chemical Society, 1992
- Isotope-filtered 2D NMR of a protein-peptide complex: study of a skeletal muscle myosin light chain kinase fragment bound to calmodulinJournal of the American Chemical Society, 1992
- HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNACell, 1991
- Assignment of the side-chain proton and carbon-13 resonances of interleukin-1.beta. using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopyBiochemistry, 1990