Suppression of Ras-Induced Transformation of NIH 3T3 Cells by Activated Gα S
- 4 March 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 263 (5151), 1278-1281
- https://doi.org/10.1126/science.8122111
Abstract
Conversion of external signals into proliferative responses may be mediated by interactions between signaling pathways that control cell proliferation. Interactions between G alpha s, the alpha subunit of the heterotrimeric guanine nucleotide binding protein that stimulates adenylyl cyclase, and Ras, an important element in growth factor signaling, were studied. Expression of activated G alpha s in NIH 3T3 cells increased intracellular concentrations of adenosine 3',5'-monophosphate (cAMP) and inhibited H-Ras-stimulated DNA synthesis and mitogen-activated protein kinase activity. Activated G alpha s and 8-Br-cAMP suppressed H-Ras-induced transformation of NIH 3T3 cells. Apparently, G alpha s inhibits proliferative signals from Ras by stimulating cAMP production and activating protein kinase A.Keywords
This publication has 22 references indexed in Scilit:
- Forging a Path to the NucleusScience, 1993
- G protein oncogenes in pituitary tumorsTrends in Endocrinology & Metabolism, 1992
- Transmembrane Receptors and Intracellular Pathways that Control Cell ProliferationAnnual Review of Physiology, 1992
- The GTPase superfamily: conserved structure and molecular mechanismNature, 1991
- Lysophosphatidate-induced cell proliferation: Identification and dissection of signaling pathways mediated by G proteinsCell, 1989
- Gasp: not just another oncogeneNature, 1989
- The cyclic AMP-mediated stimulation of cell proliferationTrends in Biochemical Sciences, 1989
- ras GENESAnnual Review of Biochemistry, 1987
- The oncogenic circle closesNature, 1982
- Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cellsNature, 1982