Prevalence and Sequence Variation of Panton-Valentine Leukocidin in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Strains in the United States
- 1 January 2012
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 50 (1), 86-90
- https://doi.org/10.1128/jcm.05564-11
Abstract
Panton-Valentine leukocidin (PVL), encoded by the lukSF-PV genes, is a putative virulence factor and marker for community-associated methicillin-resistant Staphylococcus aureus . Here we report the prevalence of PVL among a representative sample of 1,055 S. aureus infection isolates from the United States and describe the sequence variation of the lukSF-PV genes. We performed multilocus sequence typing (MLST) on all isolates and sequenced fragments of the lukSF-PV genes from a sample of 86 isolates. We assigned isolates to a PVL R or H sequence type based on a polymorphism that results in an amino acid change from arginine (R) to histidine (H). Overall, we found that 36% of S. aureus isolates were positive for lukSF-PV . Among the 86 we typed, we identified 72 R variants and 14 H variants. Among the 47 methicillin-resistance S. aureus (MRSA) isolates, 43 harbored the R variant, and among the 39 methicillin-susceptible S. aureus (MSSA) isolates, 29 harbored the R variant. Almost all (97%) of the R variants were found in MLST clonal complex 8 (CC8), while the H variant was broadly distributed among 6 CCs. Within CC8, all 38 MRSA (USA300) and all 28 MSSA isolates harbored the R variant. Of the 20 isolates from blood and the lower respiratory tract, 19 (95%) harbored the R variant. While the R variant had been linked primarily to USA300 MRSA, we found that all CC8 MSSA isolates also contained the R variant, suggesting that some strains of USA300 may have lost methicillin resistance as an adaptation in the community.Keywords
This publication has 32 references indexed in Scilit:
- Distinct Bacteriophages Encoding Panton-Valentine Leukocidin (PVL) among International Methicillin-Resistant Staphylococcus aureus Clones Harboring PVLJournal of Clinical Microbiology, 2011
- Identification and Characterization of the Multidrug Resistance Gene cfr in a Panton-Valentine Leukocidin-Positive Sequence Type 8 Methicillin-Resistant Staphylococcus aureus IVa (USA300) IsolateAntimicrobial Agents and Chemotherapy, 2010
- Community-Associated Methicillin-ResistantStaphylococcus aureus: Epidemiology and Clinical Consequences of an Emerging EpidemicClinical Microbiology Reviews, 2010
- Outbreak of Skin Infections in College Football Team Members Due to an Unusual Strain of Community-Acquired Methicillin-Susceptible Staphylococcus aureusJournal of Clinical Microbiology, 2010
- A Histidine-to-Arginine Substitution in Panton-Valentine Leukocidin from USA300 Community-Acquired Methicillin-Resistant Staphylococcus aureus Does Not Impair Its LeukotoxicityInfection and Immunity, 2010
- Augmented Production of Panton‐Valentine Leukocidin Toxin in Methicillin‐Resistant and Methicillin‐SusceptibleStaphylococcus aureusIs Associated with Worse Outcome in a Murine Skin Infection ModelThe Journal of Infectious Diseases, 2010
- Emergence and Characterization of Community-Associated Methicillin-Resistant Staphyloccocus aureus Infections in Denmark, 1999 to 2006Journal of Clinical Microbiology, 2009
- Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolates in Urban DetroitJournal of Clinical Microbiology, 2008
- A Geographic Variant of theStaphylococcus aureusPanton‐Valentine Leukocidin Toxin and the Origin of Community‐Associated Methicillin‐ResistantS. aureusUSA300The Journal of Infectious Diseases, 2008
- Fitness Cost of Staphylococcal Cassette Chromosome mec in Methicillin-Resistant Staphylococcus aureus by Way of Continuous CultureAntimicrobial Agents and Chemotherapy, 2007