In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus

Abstract

To understand the mechanisms involved in protective immunity to African swine fever virus (ASFV) infection, the observation that infection with the avirulent Portuguese ASFV isolate OUR/T88/3 protects outbred pigs from challenge with the virulent Portuguese ASFV isolate OUR/T88/1 was exploited. It was demonstrated that pigs exposed to OUR/T88/3 and then depleted of CD8⁺lymphocytes were no longer fully protected from OUR/T88/1 challenge. This indicated that CD8⁺lymphocytes play an important role in the protective immune response to ASFV infection and that anti-ASFV antibody alone, from OUR/T88/3 infection, was not sufficient to protect pigs from OUR/T88/1 challenge. Inbred pigs of the cc haplotype infected with OUR/T88/3 were not always protected from OUR/T88/1 challenge and developed both viraemia and fever. Such viraemia was always correlated with increased numbers of circulating CD8β⁺lymphocytes, indicating a specific role for CD8β⁺lymphocytes in combating viraemia. These experiments indicate an important role for CD8⁺lymphocytes, particularly CD8β⁺lymphocytes, in ASF protective immunity.