Candida albicans Adherence to Surface‐Modified Denture Resin Surfaces
- 11 July 2008
- journal article
- research article
- Published by Wiley in Journal of Prosthodontics: Implant, Esthetic, and Reconstructive Dentistry
- Vol. 17 (5), 365-369
- https://doi.org/10.1111/j.1532-849x.2007.00292.x
Abstract
The purpose of this study was to investigate two innovative methods in reducing adhesion of Candida albicans to denture base resins through modification of the surface characteristics of denture resin by incorporation of surface charge and application of a self-bonding polymer on denture resins. Three groups were tested [Group 1: control, pure poly(methyl methacrylate) (PMMA); Group 2: modified PMMA (mPMMA) with 16% methacrylic acid; Group 3: pure PMMA coated with self-bonding polymer (SBP)]. Twenty resin specimens for each group were polymerized, and four experimental subgroups for each surface type were devised, consisting of 2, 4, 6, and 12 days of incubation in C. albicans suspension. The surface area of adherent C. albicans stained with Gram's crystal violet was examined under a light microscope at 400x magnification. Four areas were photographed on each block, one on each quadrant. The images were analyzed using Scion Image 1.63 software to calculate the percent surface area containing adherent C. albicans. Kruskal-Wallis test and Tukey's honest significant difference (HSD) procedure were used to compare the groups. At day 2, the modified resin had statistically significantly lower levels of Candida than both the control group and the SBP group (p<or= 0.036). Both the mPMMA group and SBP group had statistically significantly lower levels of Candida accumulation at days 4, 6, and 12, compared to the control. The amount of C. albicans adhering to the resin surfaces reduced significantly with modification of surface charge and application of self-bonding polymer. Modification of surface characteristics of polymeric biomaterials is an effective method in reducing adhesion of C. albicans to PMMA surfaces.Keywords
This publication has 24 references indexed in Scilit:
- Influence of polymerization method, curing process, and length of time of storage in water on the residual methyl methacrylate content in dental acrylic resinsJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2005
- Serotype distribution and secretory acid proteinase activity of Candida albicans isolated from the oral mucosa of patients with denture stomatitisOral Microbiology and Immunology, 1999
- Resistance of Candida albicans biofilms to antifungal agents in vitroAntimicrobial Agents and Chemotherapy, 1995
- The influence of morphological variation on Candida albicans adhesion to denture acrylic in vitroArchives of Oral Biology, 1992
- Oral candidiasis and human immunodeficiency virus infectionJournal of Oral Pathology & Medicine, 1989
- Effect of Antibiotics on Adherence of Microorganisms to Epithelial Cell SurfacesClinical Infectious Diseases, 1985
- Oral mucosal lesions associated with the wearing of removable denturesJournal of Oral Pathology & Medicine, 1981
- Factors affecting the in-vitro adherence of Candida albicans to acrylic surfacesArchives of Oral Biology, 1980
- BACTERIAL ADHERENCE IN ORAL MICROBIAL ECOLOGYAnnual Review of Microbiology, 1975
- An epidemiologic study of yeasts in elderly denture wearersCommunity Dentistry and Oral Epidemiology, 1975