Galectin‐1 supports the survival of CD45RA(−) primary myeloma cells in vitro
Open Access
- 1 August 2008
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 142 (5), 754-765
- https://doi.org/10.1111/j.1365-2141.2008.07252.x
Abstract
The survival and proliferation of human myeloma cells are considered to be heavily dependent on the microenvironment of bone marrow (BM). This study confirmed that galectin‐1 (Gal‐1) and SDF‐1α were produced by bone marrow mononuclear cells of myeloma patients. The addition of Gal‐1 and SDF‐1α to a serum‐free synthetic medium, maintained the viability of primary myeloma cells for 2 weeks similar to that before culture. While Gal‐1 reduced the viable cell number in CD45RA(+) B cell lines, it maintained the viability of CD45(−) U266 and CD45RA(−)RO(+) ILKM3 myeloma cell lines in the synthetic medium. This was confirmed with the transfection of the PTPRC (CD45) RA, ‐RB, or ‐RO gene into CD45(−) U266 cells. The combination of Gal‐1 and SDF‐1α significantly induced phosphorylation of Akt and IkB, while the phosphorylation of ERK1/2 was significantly reduced in CD45RA(+) U266 and Raji cells but not CD45(−) or CD45RA(−) U266 cells. Furthermore, we confirmed that Gal‐1 bound to CD45RA in CD45RA(+) Raji cells, and also physically interacted with β1‐integrin by immunoprecipitation followed by Western blotting and confocal microscopy. The results suggest that Gal‐1 has two different actions depending on its binding partner, and supports the survival of CD45RA(−) myeloma cells.Keywords
This publication has 35 references indexed in Scilit:
- Galectin-1 Interacts with the α5β1 Fibronectin Receptor to Restrict Carcinoma Cell Growth via Induction of p21 and p27Journal of Biological Chemistry, 2005
- A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myelomaBlood, 2005
- Nucleocytoplasmic lectinsBiochimica et Biophysica Acta (BBA) - General Subjects, 2004
- Receptor synergy of interleukin-6 (IL-6) and insulin-like growth factor-I in myeloma cells that highly express IL-6 receptor αBlood, 2004
- Presentation of Galectin-1 by Extracellular Matrix Triggers T Cell DeathJournal of Biological Chemistry, 2004
- Galectin-1 interacts with β-1 subunit of integrinBiochemical and Biophysical Research Communications, 2003
- Upregulation of galectins‐1 and ‐3 in human colon cancer and their role in regulating cell migrationInternational Journal of Cancer, 2002
- Integrin α4β7 mediates human eosinophil interaction with MAdCAM‐1, VCAM‐1 and fibronectinImmunology, 1996
- Biphasic modulation of cell growth by recombinant human galectin-1Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1996
- Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.The Journal of Experimental Medicine, 1987