Feeding a ROS-generator to Caenorhabditis elegans leads to increased expression of small heat shock protein HSP-16.2 and hormesis
- 28 February 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Genes & Nutrition
- Vol. 4 (1), 59-67
- https://doi.org/10.1007/s12263-009-0113-x
Abstract
Reactive oxygen species (ROS) are thought to be a driving force in the aging process. In transgenic Caenorhabditis elegans expressing green fluorescent protein (GFP) under control of the hsp-16.2 promoter (CL2070) 100 muM of the ROS-generator juglone induced GFP-expression. This was associated with translocation of DAF-16 to the nucleus as visualized in a transgenic strain expressing a DAF-16::GFP fusion protein (TJ356) and with increased cellular levels of reduced glutathione. RNA-interference for DAF-16 in CL2070 blocked the juglone-induced HSP-16.2 expression and the increase in glutathione levels. Higher concentrations of juglone did not further increase the adaptive responses but caused premature death, indicating hormetic adaptations unless the stressor exceeds the intrinsic protective capacity. The addition of the ROS-scavenger ascorbic acid finally blocked lifespan reductions and all of the adaptations to juglone stressing that ROS are indeed the molecular species that require protective response.Keywords
This publication has 38 references indexed in Scilit:
- Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling methodNature Protocols, 2006
- Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatmentsBiogerontology, 2006
- Recent aging research in Caenorhabditis elegansExperimental Gerontology, 2006
- Generation of RNAi Libraries for High-Throughput ScreensJournal of Biomedicine and Biotechnology, 2006
- Transcriptional targets of DAF-16 insulin signaling pathway protectC.elegansfrom extreme hypertonic stressAmerican Journal of Physiology-Cell Physiology, 2005
- Life extension via dietary restriction is independent of the Ins/IGF-1 signalling pathway in Caenorhabditis elegansExperimental Gerontology, 2003
- Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegansNature, 2003
- Genetics and the Specificity of the Aging ProcessScience, 2003
- A C. elegans mutant that lives twice as long as wild typeNature, 1993
- Differential Regulation of Closely Related Members of thehsp16Gene Family inCaenorhabditis elegansDNA, 1989