Probiotic effects on experimental graft-versus-host disease: let them eat yogurt
- 1 June 2004
- journal article
- Published by American Society of Hematology in Blood
- Vol. 103 (11), 4365-4367
- https://doi.org/10.1182/blood-2003-11-3769
Abstract
Acute graft-versus-host disease (aGVHD) often limits feasibility and outcome of allogeneic bone marrow transplantation. Current pathophysiologic concepts of aGVHD involve conditioning regimens, donor-derived T cells, proinflammatory cytokines, and bacterial lipopolysaccharide (LPS) as a major trigger for aGVHD. LPS derives mostly from gram-negative bacteria and can enter circulation through the impaired mucosal barrier after the conditioning regimen. Probiotic microorganisms have been shown to alter the composition of the intestinal microflora and thereby mediate anti-inflammatory effects. We hypothesized that modifying the enteric flora using the probiotic microorganism Lactobacillus rhamnosus GG, would ameliorate aGVHD. Here we show that oral administration of Lactobacillus rhamnosus GG before and after transplantation results in improved survival and reduced aGVHD. Furthermore, subculturing of mesenteric lymph node tissue revealed a reduced translocation of enteric bacteria. Our findings suggest that alteration of the intestinal microflora plays an important role in the initiation of experimental aGVHD. (Blood. 2004;103:4365-4367)Keywords
This publication has 16 references indexed in Scilit:
- Rationale for Probiotic and Antibiotic Treatment Strategies in Inflammatory Bowel DiseasesDigestive Diseases, 2003
- Immunomodulatory consequences of oral administration of Lactobacillus rhamnosus strain GG in healthy volunteersJournal of Dairy Research, 2003
- Peyer's patch is the essential site in initiating murine acute and lethal graft-versus-host reactionNature Immunology, 2003
- The role of endotoxin and the innate immune response in the pathophysiology of acute graft versus host diseaseInnate Immunity, 2002
- The role of endotoxin and the innate immune response in the pathophysiology of acute graft versus host diseaseInnate Immunity, 2002
- Expression of cytokines, inducible nitric oxide synthase, and matrix metalloproteinases in pouchitis: effects of probiotic treatmentThe American Journal of Gastroenterology, 2001
- Analysis of the Fecal Microflora of Human Subjects Consuming a Probiotic Product Containing Lactobacillus rhamnosus DR20Applied and Environmental Microbiology, 2000
- Influence of Intestinal Bacterial Decontamination Using Metronidazole and Ciprofloxacin or Ciprofloxacin Alone on the Development of Acute Graft-Versus-Host Disease After Marrow Transplantation in Patients With Hematologic Malignancies: Final Results and Long-Term Follow-Up of an Open-Label Prospective Randomized TrialBlood, 1999
- Tumor necrosis factor- alpha production to lipopolysaccharide stimulation by donor cells predicts the severity of experimental acute graft-versus-host disease.JCI Insight, 1998
- Interleukin-11 promotes T cell polarization and prevents acute graft-versus-host disease after allogeneic bone marrow transplantation.JCI Insight, 1998