Oxidation of thioredoxin reductase in HeLa cells stimulated with tumor necrosis factor-α
- 3 May 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 567 (2-3), 189-196
- https://doi.org/10.1016/j.febslet.2004.04.055
Abstract
Stimulation of cells with tumor necrosis factor-α (TNF-α) results in the increase in generation of H2O2 in mitochondria that leads to apoptosis. The effect of H2O2 produced by TNF-α on the redox status of selenocysteine (SeCys) residue essential for mitochondrial thioredoxin reductase (TrxR2) was investigated in HeLa cells. TNF-α caused accumulation of oxidized TrxR2 with a thioselenide bond. The conditional induction of SeCys-deficient TrxR2 resulted in the increased production of H2O2 and apoptosis. These results suggest that the SeCys residue of TrxR2 plays a critical role in cell survival by serving as an electron donor for Trx-II and subsequent peroxiredoxin-III, which is a primary line of defense against H2O2 in mitochondriaKeywords
This publication has 50 references indexed in Scilit:
- Mitochondrial Membrane Permeabilization and Superoxide Production during ApoptosisJournal of Biological Chemistry, 2003
- Involvement of TRAF4 in Oxidative Activation of c-Jun N-terminal KinasePublished by Elsevier BV ,2002
- Essential Role of Selenium in the Catalytic Activities of Mammalian Thioredoxin Reductase Revealed by Characterization of Recombinant Enzymes with Selenocysteine MutationsJournal of Biological Chemistry, 2000
- Bridge over Troubled WatersCell, 1999
- Superoxide in ApoptosisPublished by Elsevier BV ,1998
- Laser scanning cytometry allows detection of cell death with morphological features of apoptosis in cells stained with PICytometry, 1997
- REDOX REGULATION OF CELLULAR ACTIVATIONAnnual Review of Immunology, 1997
- Efficient Reduction of Lipoamide and Lipoic Acid by Mammalian Thioredoxin ReductaseBiochemical and Biophysical Research Communications, 1996
- Cloning and sequencing of a human thioredoxin reductaseFEBS Letters, 1995
- Selenols catalyze the interchange reactions of dithiols and disulfides in waterThe Journal of Organic Chemistry, 1991